PMID: 9423862Jan 10, 1998Paper

Levels of expression and immunogenicity of attenuated Salmonella enterica serovar typhimurium strains expressing Escherichia coli mutant heat-labile enterotoxin

Infection and Immunity
M G CovoneC L Galeotti

Abstract

The effects of heterologous gene dosage as well as Salmonella typhimurium strain variability on immune response toward both the heterologous antigen, the nontoxic mutant of the Escherichia coli heat-labile enterotoxin LTK63, and the carrier Salmonella strain have been analyzed. Effects of a single integration into the host DNA and different-copy-number episomal vectors were compared in S. typhimurium delta cya delta crp delta asd strains of two different serotypes, UK-1 and SR-11. Expression of the enterotoxin in the different Salmonella isolates in vitro was found to vary considerably and, for the episomal vectors, to correlate with the plasmid copy number. LTK63-specific serum immunoglobulin G (IgG) and mucosal immunoglobulin A (IgA) antibodies were highest in mice immunized with the high-level-expression strain. High anti-LTK63 IgG and IgA titers were found to correspond to higher anti-Salmonella immunity, suggesting that LTK63 exerts an adjuvant effect on response to the carrier. Statistically significant differences in anti-LTK63 immune response were observed between groups of mice immunized with the attenuated delta cya delta crp UK-1 and SR-11 derivatives producing the antigen at the same rate. These data indicate that t...Continue Reading

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Citations

Mar 17, 2001·Scandinavian Journal of Immunology·M YamamotoH Kiyono
May 7, 2009·Immunology and Cell Biology·James E GalenMyron M Levine
Sep 22, 2009·Canadian Journal of Microbiology·André Pitondo-SilvaAna Lúcia C Darini
Dec 24, 2011·Future Microbiology·Wael Abdel Halim Hegazy, Michael Hensel
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A C KirbyM J Wick
Nov 5, 2002·FEMS Microbiology Reviews·Helen S GarmoryRichard W Titball
Oct 7, 2006·Current Opinion in Gastroenterology·E C Boedeker
Jan 25, 2011·Microbial Biotechnology·Jens KrollAlexander Steinbüchel
Sep 24, 2004·Infection and Immunity·Camille N Kotton, Elizabeth L Hohmann
Jul 2, 2003·FEMS Immunology and Medical Microbiology·Heinz P Hahn, Bernd Ulrich von Specht
Mar 23, 2000·FEMS Immunology and Medical Microbiology·D BumannT F Meyer
Jun 3, 2018·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Paola Aparecida Alves AzevedoAndré Pitondo-Silva
Mar 13, 2001·The Veterinary Journal·P MastroeniG Dougan
Dec 18, 2010·Vaccine·Vanusa P da HoraDenise L Doolan

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