Levels of germination proteins in dormant and superdormant spores of Bacillus subtilis.

Journal of Bacteriology
Sonali GhoshP Setlow

Abstract

Bacillus subtilis spores that germinated poorly with saturating levels of nutrient germinants, termed superdormant spores, were separated from the great majority of dormant spore populations that germinated more rapidly. These purified superdormant spores (1.5 to 3% of spore populations) germinated extremely poorly with the germinants used to isolate them but better with germinants targeting germinant receptors not activated in superdormant spore isolation although not as well as the initial dormant spores. The level of β-galactosidase from a gerA-lacZ fusion in superdormant spores isolated by germination via the GerA germinant receptor was identical to that in the initial dormant spores. Levels of the germination proteins GerD and SpoVAD were also identical in dormant and superdormant spores. However, levels of subunits of a germinant receptor or germinant receptors activated in superdormant spore isolation were 6- to 10-fold lower than those in dormant spores, while levels of subunits of germinant receptors not activated in superdormant spore isolation were only ≤ 2-fold lower. These results indicate that (i) levels of β-galactosidase from lacZ fusions to operons encoding germinant receptors may not be an accurate reflection ...Continue Reading

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Citations

Jul 16, 2013·Applied and Environmental Microbiology·Robyn T Eijlander, Oscar P Kuipers
Feb 19, 2013·Journal of Bacteriology·Einat SegevSigal Ben-Yehuda
Apr 30, 2013·Journal of Bacteriology·Arturo Ramirez-PeraltaPeter Setlow
Apr 24, 2014·BMC Microbiology·Elisabeth H MadslienToril Lindbäck
Feb 18, 2014·Journal of Molecular Biology·Yunfeng LiBing Hao
Mar 5, 2016·Annual Review of Food Science and Technology·Marjon H J Wells-BennikTjakko Abee
Apr 16, 2014·Molecular Microbiology·Ahasanul KobirIvan Mijakovic
Jun 12, 2014·Journal of Applied Microbiology·O M Alzahrani, A Moir
Aug 19, 2014·Research in Microbiology·Valeria Olguín-AranedaDaniel Paredes-Sabja
Nov 14, 2012·The Journal of Infectious Diseases·Jean-Philippe CorrePierre L Goossens
Nov 28, 2014·The Journal of Antimicrobial Chemotherapy·Mark J LeggettJean-Yves Maillard
Jul 28, 2020·International Journal of Molecular Sciences·Yan WangStanley Brul
Jul 13, 2017·Annual Review of Microbiology·Peter SetlowYong-Qing Li
Jan 22, 2019·Frontiers in Microbiology·Yifan Zhang, Alexander Mathys
Feb 24, 2021·International Journal of Food Microbiology·Alessia I DelbrückAlexander Mathys
Jun 24, 2016·Microbiology Spectrum·Anne Moir, Gareth Cooper
Jun 20, 2021·Comprehensive Reviews in Food Science and Food Safety·Alessia I DelbrückAlexander Mathys

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