Levels of superoxide dismutases, glutathione, and poly(ADP-ribose) polymerase in radioresistant human KB carcinoma cell line

Japanese Journal of Cancer Research : Gann
T YanagisawaJ Furuyama

Abstract

In order to investigate the radioresistance mechanism of human carcinoma cells, we measured intracellular manganese- (Mn-) and copper/zinc- (Cu/Zn-) superoxide dismutases (SODs), glutathione (GSH) and poly (ADP-ribose) polymerase (PARP) in radioresistant N10 and its parental KB cell lines. The Mn-SOD level was 1.3-fold less in N10 than in KB, but Mn-SOD was induced at 1.3 to 1.5-fold higher level in N10 than in KB by X-irradiation (4 Gy). Cu/Zn-SOD in N10 showed a higher level than that in KB both without and with irradiation. In addition, N10 had a 1.65-fold higher GSH level than did KB and became radiosensitive on treatment with buthionine sulfoximine, an inhibitor of GSH. Furthermore, PARP mRNA was highly expressed in N10 as compared to KB under unirradiated conditions. X-Irradiation reduced the PARP mRNA level in KB in a time-dependent manner, whereas the PARP mRNA level in N10 was still high at 6 h postirradiation. Assay for PARP activity demonstrated an approximately 3-fold higher activity in N10 than in KB under unirradiated conditions. X-Irradiation caused a rapid induction of PARP activity within 1 h in both cell lines, but treatment of cells with nicotinamide, a PARP inhibitor, markedly reduced the enzyme induction in...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A K GodwinM E Anderson
Sep 1, 1990·International Journal of Radiation Biology·T J McMillanJ H Peacock
May 1, 1989·Japanese Journal of Cancer Research : Gann·M UradeT Matsuya
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W R MooreA Kimura
Jan 1, 1985·Progress in Nucleic Acid Research and Molecular Biology·F Hutchinson
Oct 29, 1987·Biochemical and Biophysical Research Communications·K UchidaT Sugimura
Apr 1, 1987·Archives of Biochemistry and Biophysics·L W OberleyT D Oberley
Jul 1, 1985·Medicinal Research Reviews·I A ClarkN H Hunt
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 29, 1983·Science·A Meister
Jan 1, 1982·International Journal of Radiation Oncology, Biology, Physics·L J PetersG H Fletcher
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K HiroseK Matsushima
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M Lee, A Bernstein
Jul 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·H EAGLE

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.