Jun 9, 2020Paper

Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo

BioRxiv : the Preprint Server for Biology
C. ZengYizhou Dong

Abstract

SARS-CoV-2 has rapidly become a pandemic worldwide; therefore, an effective vaccine is urgently needed. Recently, messenger RNAs (mRNAs) have emerged as a promising platform for vaccination. Here, we systematically investigated the untranslated regions (UTRs) of mRNAs in order to enhance protein production. Through a comprehensive analysis of endogenous gene expression and de novo design of UTRs, we identified the optimal combination of 5' and 3' UTR, termed as NASAR, which was five to ten-fold more efficient than the tested endogenous UTRs. More importantly, NASAR mRNAs delivered by lipid-derived nanoparticles showed dramatic expression of potential SARS-CoV-2 antigens both in vitro and in vivo. These NASAR mRNAs merit further development as alternative SARS-CoV-2 vaccines.

Citations

Jul 17, 2020·Nature Nanotechnology·Matthew D ShinNicole F Steinmetz
Feb 4, 2021·International Journal of Nanomedicine·Dongki Yang
Jan 19, 2021·Nano Research·Wenhao ZhongJun Wu
Aug 8, 2021·Nanomaterials·Syed Mohammed Basheeruddin AsdaqKatharigatta N Venugopala
Sep 2, 2021·International Journal of Nanomedicine·Ashagrachew Tewabe YayehradAbyou Seyfu Ambaye

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