LFA-1-dependent lipid raft recruitment of DNAM-1 (CD226) in CD4+ T cell

International Immunology
Jun ShirakawaAkira Shibuya

Abstract

Upon antigen recognition by the TCR, both the leukocyte adhesion molecules DNAM-1 and leukocyte function-associated antigen-1 (LFA-1) associate with lipid rafts and form peripheral supra-molecular activation clusters that surround central-supra-molecular activation clusters at the immunological synapse. The serine residue in the cytoplasmic tail of DNAM-1 is responsible for this association of DNAM-1 with lipid rafts. The TCR-mediated signal also induces physical association of DNAM-1 with LFA-1, for which the serine phosphorylation of DNAM-1 is also responsible. However, how the serine residue is involved in lipid raft recruitment of DNAM-1 has remained unclear. Here, we show that, although the TCR-mediated signal induced the serine phosphorylation of DNAM-1, DNAM-1 did not associate with lipid rafts in CD4+ T cells derived from mice deficient in LFA-1 expression, indicating that lipid raft recruitment of DNAM-1 depends on LFA-1 expression. These results suggest that the serine phosphorylation of DNAM-1 primarily induces physical association of DNAM-1 with LFA-1, which then takes DNAM-1 into lipid raft compartment.

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Citations

Sep 9, 2015·Frontiers in Immunology·Isabelle Le MercierRandolph J Noelle
May 6, 2008·Biophysical Journal·Julia SchmitzKay-Eberhard Gottschalk
Sep 12, 2015·Brain : a Journal of Neurology·Melanie Piédavent-SalomonManuel A Friese
Apr 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monika EnqvistKarl-Johan Malmberg
Jan 19, 2010·Cellular & Molecular Immunology·Zhuwei Xu, Boquan Jin
Jun 6, 2018·Frontiers in Immunology·Hristo GeorgievGünter Bernhardt
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Liang FangBoquan Jin
Dec 18, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kelly M RamsbottomJane Oliaro
Mar 12, 2020·International Journal of Molecular Sciences·Francesca ArrugaSilvia Deaglio
Apr 9, 2020·Cancer Immunology Research·Hyung-Seung JinYoon Park

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