PMID: 9532424Apr 9, 1998Paper

LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin

Domestic Animal Endocrinology
R MamlukRina Meidan

Abstract

This study was undertaken to characterize the cDNA sequence encoding bovine LH receptor (LHR), and study the effect of different luteinization protocols on the steroidogenic capacity and LHR mRNA in bovine luteal cells. The bovine LHR cDNA sequence reported here showed high sequence identity with that of other species. Several mRNA splice variants were expressed in bovine corpus luteum (CL). Reverse transcription-polymerase chain reaction conducted with primers spanning exons 2-11 revealed, in addition to the full-length sequence, a shorter fragment homologous to splice variant B reported in porcine and ovine LHR cDNA sequences. Granulosa and theca cells derived from bovine preovulatory follicles were cultured with either forskolin (10 microM, for 8 d culture-Protocol 1) or LH (100 ng/ml, for 12 hr followed by a 7.5-d culture with 2 ng/ml-Protocol 2). LHR mRNA was not detected in luteal granulosa cells (LG); in contrast, luteal theca cells (LT) contained LHR mRNA at similar levels when cultured under Protocol 1 or 2. cAMP responses to a short challenge with LH were in good agreement with LHR mRNA levels. Cytochrome P450 side-chain-cleavage (P450scc) contents were lower in luteal cells cultured with LH as compared with cells cul...Continue Reading

References

Nov 15, 1990·Biochemical and Biophysical Research Communications·T MinegishiT ] Minegish T [corrected to Minegishi
Aug 1, 1988·Endocrine Reviews·W L Miller
Mar 1, 1988·Journal of Reproduction and Fertility·H W AlilaW Hansel
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Jul 1, 1994·Journal of Animal Science·M F SmithG W Smith
Feb 1, 1997·Biology of Reproduction·D M Stocco

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Citations

Oct 22, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Amjad R KayaniPhilip G Knight
Jun 27, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Chitra JosephRobert S Robinson
Nov 30, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Ronit YaluRina Meidan
May 30, 2013·Molecular Endocrinology·Lane K ChristensonJens Vanselow
May 5, 2011·New Zealand Veterinary Journal·K BojanicB R Jones

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