Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse

Frontiers in Neuroanatomy
Michael HeideGonzalo Alvarez-Bolado

Abstract

Acquisition of specific neuronal identity by individual brain nuclei is a key step in brain development. However, how the mechanisms that confer neuronal identity are integrated with upstream regional specification networks is still mysterious. Expression of Sonic hedgehog (Shh), is required for hypothalamic specification and is later downregulated by Tbx3 to allow for the differentiation of the tubero-mamillary region. In this region, the mamillary body (MBO), is a large neuronal aggregate essential for memory formation. To clarify how MBO identity is acquired after regional specification, we investigated Lhx5, a transcription factor with restricted MBO expression. We first generated a hypomorph allele of Lhx5-in homozygotes, the MBO disappears after initial specification. Intriguingly, in these mutants, Tbx3 was downregulated and the Shh expression domain abnormally extended. Microarray analysis and chromatin immunoprecipitation indicated that Lhx5 appears to be involved in Shh downregulation through Tbx3 and activates several MBO-specific regulator and effector genes. Finally, by tracing the caudal hypothalamic cell lineage we show that, in the Lhx5 mutant, at least some MBO cells are present but lack characteristic marker e...Continue Reading

References

Feb 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Z ShengH Westphal
Feb 1, 2000·Trends in Genetics : TIG·O Hobert, H Westphal
Mar 8, 2000·Mechanisms of Development·I Bach
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jan 7, 2004·Nature Reviews. Neuroscience·Seralynne D Vann, John P Aggleton
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Julie L Kadrmas, Mary C Beckerle
Jan 19, 2005·The European Journal of Neuroscience·Konstantin RadyushkinPeter Gruss
Jun 24, 2005·Muscle & Nerve·Boyd M KoffmanNoor A Pirzada
Sep 20, 2005·Genome Research·Belinda GiardineAnton Nekrutenko
Oct 18, 2005·Hormone Research·Aurore CaqueretJacques L Michaud
Nov 16, 2005·Annual Review of Genetics·L A NaicheVirginia E Papaioannou
Nov 18, 2005·Development·Jean-François MarionJacques L Michaud
May 3, 2007·Genes & Development·Willem M H HoogaarsVincent M Christoffels
Dec 8, 2007·Genesis : the Journal of Genetics and Development·Tianyu ZhaoGonzalo Alvarez-Bolado
Dec 3, 2008·The European Journal of Neuroscience·Tianyu ZhaoGonzalo Alvarez-Bolado
Jan 20, 2009·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Michael D KopelmanE Jane Marshall
Feb 19, 2009·Development, Growth & Differentiation·Takeshi Shimizu, Masahiko Hibi
Mar 3, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nataliya BorodovskyGil Levkowitz
Mar 12, 2009·Methods : a Companion to Methods in Enzymology·Dominic SchmidtDuncan T Odom
May 12, 2009·Nature Biotechnology·Cole Trapnell, Steven L Salzberg
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nora-Emöke SzabóGonzalo Alvarez-Bolado
May 4, 2010·Nature Neuroscience·Tomomi ShimogoriSeth Blackshaw
Jun 15, 2010·Trends in Neurosciences·Steffen Scholpp, Andrew Lumsden
Aug 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amaya MiquelajáureguiYangu Zhao
Aug 10, 2010·BMC Bioinformatics·Mali Salmon-DivonPaul Bertone
Nov 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Li ChenJames Y H Li
Mar 11, 2011·Annals of the New York Academy of Sciences·Yossy MachlufGil Levkowitz
Apr 19, 2011·Cardiovascular Research·Franziska GreulichAndreas Kispert
May 6, 2011·Bioinformatics·Timothy L Bailey
Nov 9, 2012·Development·Mallika ChatterjeeJames Y H Li

❮ Previous
Next ❯

Citations

May 6, 2019·Journal of Neuroendocrinology·Travis FuMarysia Placzek
Nov 19, 2015·Frontiers in Neuroanatomy·Amaya MiquelajáureguiAlfredo Varela-Echavarría
Dec 17, 2020·Frontiers in Neuroanatomy·Carmen Diaz, Luis Puelles
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Lara López-GonzálezLuis Puelles

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE61614

Methods Mentioned

BETA
ChIP-Seq
PCR
ICE
Chip
Transfection
immunoprecipitation
ChIPseq

Software Mentioned

ImageJ
PeakAnalyzer
DREME
Bowtie
Peak Annotation with Peak Analyzer
Galaxy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Nora-Emöke SzabóGonzalo Alvarez-Bolado
Frontiers in Neuroanatomy
Sandra BlaessGonzalo Alvarez-Bolado
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Seth BlackshawTomomi Shimogori
Seminars in Cell & Developmental Biology
James Briscoe, Johan Ericson
© 2021 Meta ULC. All rights reserved