PMID: 336538Nov 1, 1977

Liability to hydrophobic and charge interaction of smooth Salmonella typhimurium 395 MS sensitized with anti-MS immunoglobulin G and complement

Infection and Immunity
I StjernströmC Tagesson

Abstract

Sensitization of smooth Salmonella typhimurium 395 MS bacteria with hyper-immune anti-MS immunoglobulin G (IgG) antibodies increased the liability to hydrophobic interaction as assessed by the affinity for a column of Octyl-Sepharose. After sensitization, the material originally eluted with 1 M (NH4)SO4 in a 0.01 M phosphate buffer (pH 6.8) was not desorbed until the ionic strength was reduced to nil, and 0.1% (vol/vol) Triton X-100 in the 0.01 M phosphate buffer was used as eluant. Furthermore, by including positively charged bis-trimethylamino-polyethylene glycol (PEG) or negatively charged bis-sulfoamino-PEG in an aqueous two-phase system of dextran T500 and PEG 6000, the partition of the IgG-sensitized bacteria was affected by either of the polymers, whereas that of the parent bacteria was not. The hydrophobic effect of IgG binding was enhanced by complement. With heat-inactivated complement, the effect of IgG was diminished. The F(ab')2 fragment showed a much lower capacity to promote a hydrophobic interaction than the complete IgG molecule.

Citations

Apr 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·L OhmanO Stendahl
Jan 1, 1980·Scandinavian Journal of Infectious Diseases. Supplementum
Apr 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·O StendahlL Edebo
Dec 1, 1979·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·S ColleenP A Mårdh
Apr 1, 1978·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·K E MagnussonL Edebo
Apr 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·L OhmanO Stendahl

Related Concepts

Antibodies, Bacterial
Chromatography, Affinity
Hemolytic Complement
Polyglobin
Salmonella typhimurium LT2
Surface Properties

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