Library screening for D-amino-acid oxidase gene: application of real-time PCR

Amino Acids
Ryuichi Konno, A Niwa

Abstract

Quantitative real-time PCR shows the quantity in addition to the presence of the target sequence. This property seemed very useful for library screening. Then, real-time PCR was employed to screen for lambda phages carrying D-amino-acid oxidase gene from mouse genomic library. Using stepwise dilution screening combined with real-time PCR, positive phages were isolated in a short time.

Citations

Sep 1, 2011·Chemical Reviews·Fien Van LanckerNorbert De Kimpe

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