Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus

Scientific Reports
Keiji NumataMisato Ohtani

Abstract

Cell-penetrating peptides (CPPs) are used for various applications, especially in the biomedical field. Recently, CPPs have been used as a part of carrier to deliver proteins and/or genes into plant cells and tissues; hence, these peptides are attractive tools for plant biotechnological and agricultural applications, but require more efficient delivery rates and optimization by species before wide-scale use can be achieved. Here, we developed a library containing 55 CPPs to determine the optimal CPP characteristics for penetration of BY-2 cells and leaves of Nicotiana benthamiana, Arabidopsis thaliana, tomato (Solanum lycopersicum), poplar (hybrid aspen Populus tremula × tremuloides line T89), and rice (Oryza sativa). By investigating the cell penetration efficiency of CPPs in the library, we identified several efficient CPPs for all the plants studied except rice leaf. In the case of rice, several CPPs showed efficient penetration into rice callus. Furthermore, we examined the relationship between cell penetration efficiency and CPP secondary structural characteristics. The cell penetration efficiency of Lys-containing CPPs was relatively greater in plant than in animal cells, which could be due to differences in lipid composi...Continue Reading

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Citations

Oct 8, 2020·Biomaterials Science·Kübra Kaygisiz, Christopher V Synatschke
Jan 15, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Neval YilmazKeiji Numata
Apr 27, 2021·ACS Biomaterials Science & Engineering·Kenta WatanabeKeiji Numata
Apr 2, 2021·Bioscience, Biotechnology, and Biochemistry·Yoshino TanakaTakashi Iwasaki
Jul 8, 2021·Plant & Cell Physiology·Noriyoshi YagiMasayoshi Nakamura
Apr 23, 2019·ACS Synthetic Biology·Md Monirul IslamKeiji Numata
Aug 31, 2021·RSC Chemical Biology·Keiko MidorikawaKeiji Numata
Oct 27, 2021·Advanced Materials·Yong YanFeng Wang

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Methods Mentioned

BETA
circular dichroism
phosphotransferase

Software Mentioned

DichroWeb
SELCON3
SPSS Statistics

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