Abstract
Licochalcone A (LCA), isolated from the root of Glycyrrhiza inflata, are known to have medicinal effect such as anti-oxidant, anti-bacterial, anti-viral, and anti-cancer. Though, as a pharmacological mechanism regulator, anti-cancer studies on LCA were not investigated in human breast cancer. We investigated the anti-proliferative and apoptotic effect of LCA in human breast cancer cells MCF-7 and MDA-MB-231 through MTS assay, PI staining, Annexin-V/7-AAD assay, mitochondrial membrane potential assay, multi-caspase assay, RT-PCR, Western blot analysis, and anchorage-independent cell transformation assay. Our results showed the little difference between two cells, as MCF-7 cell is both estrogen/progesterone receptor positive, there were only effect on Sp1 protein level, but not in mRNA level. Adversely, estrogen/progesterone/human epidermal growth factor receptor 2 triple negative, MDA-MB-231 showed decreased Sp1 mRNA, and protein levels. To confirm the participation of Sp1 in breast cancer cell viability, siRNA techniques were introduced. Both cells showed dysfunction of mitochondrial membrane potential and mitochondrial ROS production, which reflects it passed intracellular mitochondrial apoptosis pathway. Additionally, LCA sho...Continue Reading
References
Jan 1, 1994·Stem Cells·S Shibata
Feb 20, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P A GanzG E Wyatt
Aug 28, 1998·Science·D R Green, J C Reed
Mar 27, 2001·Gynecologic Oncology·D A LevineJ Boyd
Aug 21, 2003·Breast Cancer Research : BCR·Ruth C Travis, Timothy J Key
Feb 8, 2005·BMJ : British Medical Journal·Caroline BurgessAmanda Ramirez
Jul 13, 2005·Journal of Ethnopharmacology·Gordon M Cragg, David J Newman
May 23, 2006·Oncogene·E DeniaudY Leverrier
Nov 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masashi KanaiKeping Xie
Dec 7, 2007·Biological & Pharmaceutical Bulletin·Hee Jung ChangYoung Do Jung
Dec 13, 2007·Journal of Cell Science·Susan Chalmers, John G McCarron
Feb 6, 2008·The Veterinary Clinics of North America. Small Animal Practice·Ling GaoHua Cai
Nov 14, 2008·Journal of Natural Products·Rostand Manfouo NgoumfoMuhammad Iqbal Choudhary
Dec 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mitch Dowsett, Anita K Dunbier
Jul 10, 2009·International Journal of Cancer. Journal International Du Cancer·Jian-Ying ChuangJan-Jong Hung
Feb 18, 2010·International Journal of Molecular Sciences·Ewelina SzliszkaWojciech Krol
Apr 3, 2012·European Journal of Pharmacology·Chung Soo LeeJeong Jae Lee
Jul 24, 2013·International Journal of Oncology·Dong-Wook KimJung-Hyun Shim
Aug 13, 2013·BioMed Research International·Xuan YuanQiusheng Zheng
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jan 28, 2014·PloS One·Jen-Pi TsaiYi-Hsien Hsieh
Apr 3, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guang ZengWenxing Xun
May 6, 2014·Expert Opinion on Therapeutic Targets·Stephen SafeIndira Jutooru
May 27, 2014·International Journal of Oncology·Jung Jae ChoJung-Hyun Shim
Aug 26, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hung-Che HuangYi-Hsien Hsieh
Nov 14, 2014·The FEBS Journal·Kate Beishline, Jane Azizkhan-Clifford
Nov 25, 2014·Phytotherapy Research : PTR·María I TracannaHoracio Bach
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 13, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mi-Ra ParkJae-Sung Kim
Feb 25, 2015·The British Journal of Ophthalmology·Zeynep DadaciMehmet Borazan
Mar 24, 2015·Obstetrics & Gynecology Science·Sang Eun OhMin Kyu Kim
May 20, 2015·Scientific Reports·Wenjin HaoQiusheng Zheng
Aug 28, 2015·Oncotarget·Jen-Pi TsaiYi-Hsien Hsieh
Oct 16, 2015·BMC Research Notes·Paolo ZuccaAndrea C Rinaldi
Oct 16, 2015·PloS One·Natalia NowackaAnna Malm
Oct 30, 2015·CA: a Cancer Journal for Clinicians·Carol E DeSantisAhmedin Jemal
Nov 5, 2015·Oncology Reports·Zhiping HeYi Charlie Chen
Dec 8, 2015·CA: a Cancer Journal for Clinicians·Carolyn D RunowiczPatricia A Ganz
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Nov 7, 2016·The Breast : Official Journal of the European Society of Mastology·H J G AbrahamsH Knoop
Citations
Apr 24, 2018·Phytotherapy Research : PTR·Bo Deng, Wei Sun
Aug 8, 2018·Food & Function·Wan Jung LuKuan Hung Lin
Mar 8, 2019·Cells·Wen-Chung HuangChian-Jiun Liou
May 18, 2019·Recent Patents on Anti-cancer Drug Discovery·Chuanjun QuDefang Li
Dec 30, 2017·Oncotarget·Yi-Wei SunXin Che
Jan 20, 2018·Scientific Reports·Rong LiuCeshi Chen
Jul 28, 2018·Mini Reviews in Medicinal Chemistry·Gatta Daniela Maria PiaSperanza Lorenza
Mar 13, 2019·Frontiers in Immunology·Wenjin GuoJuxiong Liu
Aug 8, 2019·International Journal of Molecular Sciences·Su Hyun HongYung Hyun Choi
Nov 28, 2020·The Biochemical Journal·Shuai GongDonghang Cheng
Apr 30, 2020·Journal of Natural Products·Chung-Pu WuSuresh V Ambudkar
Sep 12, 2018·Journal of Proteome Research·Catherine C GoingSharon J Pitteri
Dec 7, 2021·Natural Product Research·Paloma Silva de SouzaMichelle Frazão Muzitano
Oct 24, 2020·Current Topics in Medicinal Chemistry·Jiaqi XiaoFeng Gao
Dec 8, 2021·Biomolecules & Therapeutics·Kyung-Ho ParkJung-Hyun Shim