Lidocaine increases intracellular sodium concentration through a Na+-H+ exchanger in an identified Lymnaea neuron

Anesthesia and Analgesia
Shin OnizukaMayumi Takasaki

Abstract

The intracellular sodium concentration ([Na(+)]in) is related to neuron excitability. For [Na(+)]in, a Na(+)-H(+) exchanger plays an important role, which is affected by intracellular pH ([pH]in). However, the effect of lidocaine on [pH]in and a Na(+)-H(+) exchanger is unclear. We used neuron from Lymnaea stagnalis to determine how lidocaine affects [pH]in, Na(+)-H(+) exchanger, and [Na(+)]in. Intracellular sodium imaging by sodium-binding benzofuran isophthalate and intracellular pH imaging by 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein were used to measure [Na(+)]in and [pH]in. Measurements for [Na(+)]in were made in normal, Na(+) free saline, with modified extracellular pH, and a Na(+)-H(+) exchanger antagonist [(5-N-ethyl-N-isopropyl amiloride, N-methylisopropylamiloride, and 5-(N,N-hexamethylene)-amiloride) pretreatment trials. Furthermore, [Na(+)]in and [pH]in were recorded simultaneously. From 0.1 to 10 mM, lidocaine, mepivacaine, bupivacaine, prilocaine, and QX-314 were evaluated. Lidocaine, mepivacaine, and prilocaine increased the [Na(+)]in in a dose-dependent manner. In contrast, QX-314 did not change the [Na(+)]in at each dose. In the Na(+) free saline or in the presence of each Na(+)-H(+) exchanger antagonist...Continue Reading

References

Apr 11, 1989·The American Journal of Physiology·M E GerritsenM S Medow
Feb 1, 1989·The Journal of Physiology·M S Szatkowski, R C Thomas
Oct 1, 1982·The Journal of Cell Biology·T J RinkT Pozzan
Sep 15, 1994·The Journal of Physiology·G J Cooper, M Hunter
Oct 1, 1994·Anesthesia and Analgesia·M H Kim-LeeJ S McDonald
Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R C DukeD M Ojcius
Mar 15, 1997·Experimental Cell Research·M KimR D Wurster
May 12, 1998·The American Journal of Physiology·T N Nagaraja, N Brookes
Aug 12, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J I GoldsteinJ C Leiter
Feb 28, 2002·The Journal of Biological Chemistry·Yoko AraiMinoru Kasuya
Jul 2, 2003·European Journal of Medicinal Chemistry·B MasereelD Laeckmann
Nov 7, 2003·Journal of Neurophysiology·Anna K VinnikovaVijay Lyall
Jun 15, 2004·Cell Death and Differentiation·D Lagadic-GossmannV Lecureur
Mar 10, 2005·American Journal of Physiology. Renal Physiology·Craig BrooksZheng Dong
Apr 16, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Khalid AbdounHolger Martens
Apr 14, 2007·Cell Death and Differentiation·D N CriddleO V Gerasimenko
Jan 19, 1990·Science·R A Kerr
Oct 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juan D Goutman, Elisabeth Glowatzki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.