Ligand- and DNA-induced dissociation of RXR tetramers

Journal of Molecular Biology
Z P ChenH Gronemeyer

Abstract

Unliganded bacterially expressed RXR alpha lacking the N-terminal region AB (apo-RXR alpha delta AB) was found in solution as an apparent mixture of 165 kDa tetramers and 42 kDa monomers which could be quantitatively separated by gel filtration and non-denaturing gel electrophoresis. Under identical conditions both liganded (holo-) and apo-RAR alpha delta AB were present as single monomeric species. apo-RXR alpha delta AB tetramers, as well as dimers of the apo-RXR ligand binding domain (apo-LBD), dissociated readily into monomers when exposed to their cognate ligand 9-cis retinoic acid (9c-RA). The apo-RXR alpha delta AB tetramer bound only transiently to a cognate DR1 response element, and was converted into DR1-apo-RXR alpha delta AB homodimer complexes indistinguishable from those generated by cooperative DNA binding of apo-RXR alpha delta AB monomers. In the absence of DNA, the addition of 9c-RA greatly accelerated the formation of heterodimers with the apo-RAR alpha delta AB heterodimerization partner. No RXR alpha delta AB or RAR alpha delta AB homodimers could be observed in solution, but upon mixing of the two receptor monomers stable heterodimers could be isolated which bound to DR5 response elements in a highly coope...Continue Reading

References

Dec 18, 1992·Science·J M LehmannM Pfahl
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S KerstenN Noy
May 18, 1995·Nature·H Gronemeyer, D Moras
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Aug 29, 1996·Nature·J Y ChenH Gronemeyer
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C LiR M Evans

❮ Previous
Next ❯

Citations

Nov 3, 2004·Nature Reviews. Drug Discovery·Hinrich GronemeyerVincent Laudet
May 1, 2002·The EMBO Journal·Paul L Shaffer, Daniel T Gewirth
May 24, 2011·Nucleic Acids Research·Jose M G Vilar, Leonor Saiz
Jan 7, 2010·PPAR Research·Lap Shu Alan Chan, Richard A Wells
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yan ChenJoachim D Müller
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·W W PijnappelA J Durston
Mar 12, 2013·Journal of Medicinal Chemistry·Martin Conda-SheridanMark Cushman
Jan 21, 2017·Cellular and Molecular Life Sciences : CMLS·María P Menéndez-Gutiérrez, Mercedes Ricote
Oct 25, 2002·Journal of Cellular Biochemistry·Quentin Campbell HewsonChristopher P F Redfern
Jul 12, 2002·Journal of Cellular Biochemistry·Birju RanaChristopher P F Redfern
Dec 21, 2004·Cell Death and Differentiation·A SzantoL Nagy
Apr 4, 2019·Medicinal Research Reviews·Nathalia Rodrigues de Almeida, Martin Conda-Sheridan
Apr 27, 2019·Molecular and Cellular Endocrinology·Wojciech KrężelAngel R de Lera
Nov 13, 1998·Journal of Molecular Biology·S KerstenN Noy
Jul 27, 1999·Protein Expression and Purification·J IyerH Gronemeyer
Jun 2, 2021·Journal of the Royal Society, Interface·Pankaj Gautam, Sudipta Kumar Sinha
Aug 9, 2005·Journal of the American Society for Mass Spectrometry·Johan LengqvistWilliam J Griffiths

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life
Kunihiko Kaneko
Biochemical and Biophysical Research Communications
A M Christianson, F C Kafatos
© 2021 Meta ULC. All rights reserved