Ligand binding and covalent structure of an oxygen-binding heme protein from Rhodobacter sphaeroides, a representative of a new structural family of c-type cytochromes

Biochemistry
K KlarskovJ Van Beeumen

Abstract

The amino acid sequence of an oxygen-binding heme protein (SHP) from Rhodobacter sphaeroides has been determined. The cysteines, which bind the single heme group in the 112-residue protein, are located at positions 43 and 46. SHP is similar in size to the large membrane-bound form of the class I cytochrome c5 of Azotobacter vinelandii (116 residues) and in the location of the heme binding site at positions 48 and 51. Two extra cysteines in SHP (residues 89 and 97) are located in positions similar to those of cytochrome c5 (residues 98 and 101) and form a disulfide bridge in both proteins. In total, four regions of alpha-helix are predicted, covering 46% of the protein, which is comparable to that in other small cytochromes. SHP is thus distantly related to small class I c-type cytochromes but is representative of a distinct family by virtue of its high-spin nature, the lack of a strong sixth ligand, and its capacity to bind oxygen. Potentially, the most important characteristic of SHP is its ability to transiently bind oxygen during autoxidation, which occurs with a half-life of 3 min with a 4-fold excess of O2. SHP also binds carbon monoxide, azide, and cyanide. The kinetics of reduction by free flavins indicate that SHP is le...Continue Reading

References

Jul 20, 1985·Journal of Molecular Biology·D C CarterC D Stout
Sep 20, 1985·Biochimica Et Biophysica Acta·R J KassnerM A Cusanovich
Mar 1, 1973·The Biochemical Journal·R P Ambler, M Wynn
Jan 15, 1994·European Journal of Biochemistry·B SamynJ J van Beeumen

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Citations

Nov 26, 2008·Natural Product Reports·Sarah E J Bowman, Kara L Bren
Aug 5, 2017·Journal of Clinical Pathology·Joe Lee, Claire N Harrison
Oct 25, 2000·Protein Science : a Publication of the Protein Society·D ZhaoM A Cusanovich
Sep 17, 2008·Biochemical Society Transactions·Bor-Ran LiStephen K Chapman
May 24, 2000·The Journal of Biological Chemistry·D LeysJ J Van Beeumen
Jan 13, 2006·Chemical Reviews·Ivano BertiniAntonio Rosato

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