Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures.

The Journal of Biological Chemistry
Josie A SilvaroliMarcin Golczak

Abstract

Important in regulating the uptake, storage, and metabolism of retinoids, cellular retinol-binding protein 1 (CRBP1) is essential for trafficking vitamin A through the cytoplasm. However, the molecular details of ligand uptake and targeted release by CRBP1 remain unclear. Here we report the first structure of CRBP1 in a ligand-free form as well as ultra-high resolution structures of this protein bound to either all-trans-retinol or retinylamine, the latter a therapeutic retinoid that prevents light-induced retinal degeneration. Superpositioning of human apo- and holo-CRBP1 revealed major differences within segments surrounding the entrance to the retinoid-binding site. These included α-helix II and hairpin turns between β-strands βC-βD and βE-βF as well as several side chains, such as Phe-57, Tyr-60, and Ile-77, that change their orientations to accommodate the ligand. Additionally, we mapped hydrogen bond networks inside the retinoid-binding cavity and demonstrated their significance for the ligand affinity. Analyses of the crystallographic B-factors indicated several regions with higher backbone mobility in the apoprotein that became more rigid upon retinoid binding. This conformational flexibility of human apo-CRBP1 facilita...Continue Reading

References

Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C SacchettiniL J Banaszak
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E LiJ I Gordon
Jan 1, 1983·Advances in Nutritional Research·F Chytil, D E Ong
Apr 20, 1993·Journal of Molecular Biology·N S WinterL J Banaszak
Sep 16, 1999·The EMBO Journal·N B GhyselinckP Chambon
Feb 10, 2000·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·C FolliR Berni
Apr 6, 2002·The Journal of Biological Chemistry·Lorella FranzoniHeinz Rüterjans
Aug 7, 2002·Journal of Molecular Biology·Vito CalderoneGiuseppe Zanotti
Aug 15, 2002·The Journal of Biological Chemistry·Claudia FolliRodolfo Berni
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 14, 2004·Biochimica Et Biophysica Acta·Federico TortaGian Luigi Rossi
May 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Marcin GolczakKrzysztof Palczewski
Oct 12, 2005·The Journal of Biological Chemistry·Marcin GolczakKrzysztof Palczewski
May 12, 2006·Journal of Neurobiology·Rune Blomhoff, Heidi Kiil Blomhoff
Jul 29, 2008·The Journal of Biological Chemistry·Akiko MaedaKrzysztof Palczewski
Nov 21, 2009·Trends in Biochemical Sciences·Vytas A BankaitisGabriel Schaaf
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Jul 22, 2011·ACS Chemical Biology·Riki KawaguchiHui Sun
Feb 27, 2013·Journal of Lipid Research·Ziad Al TanouryCécile Rochette-Egly
Apr 30, 2013·Journal of Lipid Research·Sheila M O'Byrne, William S Blaner
Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera
Sep 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Bohdan SchneiderJiří Černý
Sep 30, 2014·Traffic·Nozomu Kono, Hiroyuki Arai
Nov 11, 2014·Nature Chemical Biology·Marcin GolczakKrzysztof Palczewski
Dec 6, 2014·Acta Crystallographica. Section D, Biological Crystallography·Zahra NossoniJames Geiger

❮ Previous
Next ❯

Citations

Oct 30, 2016·Nutrients·Sylwia ChelstowskaMarcin Golczak
Oct 26, 2018·The Biochemical Journal·Anahita DaruwallaPhilip D Kiser
Oct 26, 2018·Proteomics·Etienne VillainAndrey V Kajava
Jul 7, 2018·Scientific Reports·Kasun RatnayakeAjith Karunarathne
May 30, 2020·Critical Reviews in Biochemistry and Molecular Biology·William S BlanerMarcin Golczak
Aug 14, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Yi-Yun ChengPing-Chiang Lyu
Nov 13, 2019·The Journal of Physical Chemistry Letters·Carolina EstarellasF Javier Luque
Nov 27, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Made Airanthi K Widjaja-Adhi, Marcin Golczak
Dec 24, 2020·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Guo ZhongNina Isoherranen
Feb 26, 2021·Journal of Lipid Research·Josie A SilvaroliMarcin Golczak
Mar 24, 2018·Archives of Biochemistry and Biophysics·Ilaria MenozziRodolfo Berni
May 12, 2021·The Journal of Physical Chemistry. B·Patrice KoehlHenri Orland
Feb 6, 2019·ACS Chemical Biology·Josie A SilvaroliMarcin Golczak

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.