PMID: 213175Dec 15, 1978

Ligand binding studies in the mouse olfactory bulb: identification and characterization of a L-[3H]carnosine binding site

Brain Research
J D HirschF L Margolis

Abstract

Binding sites for the dipeptide L-carnosine (beta-alanyl-L-histidine) have been detected in membranes prepared from mouse olfactory bulbs. The binding of L-[3H]-carnosine was saturable, reversible and stereospecific and had a Kd of about 770 nM. The stereospecific binding of L-carnosine represented about 30% of the total binding at pH 6.8, and decreased markedly with increasing pH. Binding was stimulated by calcium, unaffected by zinc, magnesium or manganese and inhibited by sodium and potassium. Carnosine binding was sensitive to trypsin and phospholipases A and C, but not to neuraminidase. Nystatin and filipin, which interact with membrane lipids, also interferred with binding. Some peptide analogues of carnosine were potent inhibitors of binding, but a variety of drugs serving as potent inhibitors in other binding systems had no effect on carnosine binding. Carnosine binding to mouse olfactory bulb membranes was 15-fold higher than that seen in membranes prepared from cerebral hemispheres, 5-fold higher than that seen in membranes prepared from cerebral hemispheres, 5-fold higher than in cerebellum membranes and 3-fold higher than in membranes from spinal medulla and the olfactory tubercle-lateral olfactory tract area. Bindi...Continue Reading

References

Jan 1, 1976·Annual Review of Physiology·S H Snyder, J P Bennett
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Citations

Dec 8, 1980·Brain Research·M T González-Estrada, W J Freeman
May 17, 1982·Life Sciences·J E Seely, F D Marshall
May 18, 1983·Biochimica Et Biophysica Acta·F L MargolisA I Farbman
Nov 1, 1983·Neuroscience·N Halász, G M Shepherd
May 1, 1992·Brain Research Bulletin·M J Crowe, S K Pixley
Sep 29, 1999·Progress in Neurobiology·L BonfantiA Fasolo
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D P Taylor, C B Pert
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C B Srikant, Y C Patel
Aug 1, 1990·Journal of Neurochemistry·J J O'DowdD J Miller
Jan 1, 1982·Scandinavian Journal of Immunology. Supplement·F L Margolis

Related Concepts

Carnosine, (D-His)-Isomer
Denervation
Dipeptides
Dose-Response Relationship, Drug
Electrolytes
Accessory Olfactory Bulb
Radioligand Assay
Molecular Stereochemistry
Synaptic Receptors

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