Ligand Diffusion Enables Force-Independent Cell Adhesion via Activating α5β1 Integrin and Initiating Rac and RhoA Signaling.

Advanced Materials
Leixiao YuRainer Haag

Abstract

Cells reside in a dynamic microenvironment in which adhesive ligand availability, density, and diffusivity are key factors regulating cellular behavior. Here, the cellular response to integrin-binding ligand dynamics by directly controlling ligand diffusivity via tunable ligand-surface interactions is investigated. Interestingly, cell spread on the surfaces with fast ligand diffusion is independent of myosin-based force generation. Fast ligand diffusion enhances α5β1 but not αvβ3 integrin activation and initiates Rac and RhoA but not ROCK signaling, resulting in lamellipodium-based fast cell spreading. Meanwhile, on surfaces with immobile ligands, αvβ3 and α5β1 integrins synergistically initiate intracellular-force-based canonical mechanotransduction pathways to enhance cell adhesion and osteogenic differentiation of stem cells. These results indicate the presence of heretofore-unrecognized pathways, distinct from canonical actomyosin-driven mechanisms, that are capable of promoting cell adhesion.

References

Apr 27, 2000·Nature Cell Biology·E ZamirB Geiger
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Aug 12, 2005·Chemical Reviews·Martin K Beyer, Hauke Clausen-Schaumann
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Nov 1, 2007·Nature Reviews. Molecular Cell Biology·Mark R MorganMark D Bass
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pere Roca-CusachsMichael P Sheetz
May 31, 2011·Biomaterials·Andrew R CameronJustin J Cooper-White
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Dec 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Cheng-han YuMichael P Sheetz
Aug 15, 2012·Chemical Society Reviews·Eric A AppelOren A Scherman
Jul 31, 2013·Stem Cells·Andrew W HolleAdam J Engler
Mar 22, 2014·Biomaterials·Andreas P KourouklisHarry Bermudez
Apr 8, 2014·Biophysical Journal·Carol JurchenkoKhalid S Salaita
Jul 22, 2014·Angewandte Chemie·Qiang WeiRainer Haag
Oct 31, 2014·Nature Reviews. Molecular Cell Biology·Jay D HumphreyMartin A Schwartz
Nov 22, 2014·Nature Reviews. Molecular Cell Biology·Caroline BonnansZena Werb
Feb 20, 2015·Nature Communications·Ovijit ChaudhuriDavid J Mooney
Jul 2, 2015·Nature Reviews. Molecular Cell Biology·Michael MurrellMargaret L Gardel
Dec 1, 2015·Nature Materials·Ovijit ChaudhuriDavid J Mooney
Jun 4, 2016·Angewandte Chemie·Carlos Mas-MorunoHorst Kessler
Jan 28, 2017·Nature Communications·Mitasha BharadwajDaniel J Müller
May 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·William J HaddenYu Suk Choi
Sep 28, 2017·Scientific Reports·Stéphanie M C BruekersWilhelm T S Huck
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ming GuoDavid A Weitz
Nov 9, 2017·Nature Reviews. Molecular Cell Biology·Kyle H Vining, David J Mooney
Nov 22, 2017·Accounts of Chemical Research·Yang LiuKhalid Salaita
Dec 1, 2017·ACS Applied Materials & Interfaces·Leixiao YuRainer Haag
Dec 7, 2017·Nature·Roger OriaPere Roca-Cusachs
Feb 7, 2018·Nature Cell Biology·Jau-Ye ShiuViola Vogel
Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ze GongVivek B Shenoy
Nov 8, 2018·Annual Review of Physiology·Haguy WolfensonMichael P Sheetz
Jan 4, 2019·Nature Communications·Maksim KunitskiReinhard Dörner
Mar 5, 2019·ACS Nano·Catherine S HanselMolly M Stevens

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Citations

Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Qing Zhang
May 14, 2021·Bioinorganic Chemistry and Applications·Weiping ZhengChangqing Yuan
Aug 19, 2021·Nano Research·Shuang ZhengJiandong Ding
Jan 19, 2021·ACS Biomaterials Science & Engineering·Qiang WeiElisabetta A Cavalcanti-Adam
Nov 21, 2020·ACS Nano·Stephanie A MaynardMolly M Stevens

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