Ligand dynamics in heme proteins observed by Fourier transform infrared-temperature derivative spectroscopy

Biochimica Et Biophysica Acta
Karin Nienhaus, G Ulrich Nienhaus

Abstract

Fourier transform infrared (FTIR) spectroscopy is a powerful tool for the investigation of protein-ligand interactions in heme proteins. Nitric oxide and carbon monoxide are attractive physiologically relevant ligands because their bond stretching vibrations give rise to strong mid-infrared absorption bands that can be measured with exquisite sensitivity and precision using photolysis difference spectroscopy at cryogenic temperatures. These stretching bands are fine-tuned by electrostatic interactions with the environment and, therefore, ligands can be utilized as local probes of structure and dynamics. Bound to the heme iron, the ligand stretching bands are susceptible to changes in the iron-ligand bond and the electric field at the active site. Upon photolysis, the vibrational bands display changes due to ligand relocation to docking sites within the protein, rotational motions of the ligand in these sites and protein conformational changes. Photolysis difference spectra taken over a wide temperature range (3-300K) using specific temperature protocols for sample photodissociation can provide detailed insights into both protein and ligand dynamics. Moreover, temperature-derivative spectroscopy (TDS) has proven to be a particul...Continue Reading

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Citations

Jun 5, 2013·Physical Chemistry Chemical Physics : PCCP·Stefania AbbruzzettiCristiano Viappiani
Sep 22, 2009·The Journal of Biological Chemistry·Elena NickelG Ulrich Nienhaus
Oct 9, 2008·The Journal of Biological Chemistry·Mallory D SalterJohn S Olson
Feb 18, 2016·Analytical and Bioanalytical Chemistry·Ángela I López-Lorente, Boris Mizaikoff
May 24, 2011·Biochimica Et Biophysica Acta·Stefano BettatiCristiano Viappiani
Apr 16, 2013·Biochimica Et Biophysica Acta·Francesca SpyrakisF Javier Luque
Feb 8, 2013·Biochimica Et Biophysica Acta·M Fátima Lucas, Víctor Guallar
Aug 30, 2008·PLoS Pathogens·Anthony W MaressoOlaf Schneewind
Oct 15, 2011·Bioresource Technology·Shefali SangarRavinder S Jolly
Feb 9, 2017·Chemical Reviews·Ilia G Denisov, Stephen G Sligar
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria
Jul 18, 2017·The Journal of Physical Chemistry. B·Karin NienhausG Ulrich Nienhaus

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