Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles.

Journal of Chromatography. a
Lin-Sen QingaYi-Ming Liu

Abstract

Dioscorea nipponica and the preparations made from it have been used for long to prevent and treat coronary heart disease in traditional Chinese medicine. A group of steroidal saponins present in the plant are believed to be the active ingredients. It has been a challenge to study the individual saponins separately due to the similarities in their chemical and physical properties. In this work, human serum albumin (HSA) functionalized magnetic nanoparticles (MNPs) were used to isolate and identify saponin ligands that bind to HSA from D. nipponica extract. Electrospray ionization mass spectrometry (ESI-MS) was used for compound identification and semi-quantification. Three saponins, i.e. dioscin, gracillin, and pseudo-protodioscin showed affinity to HSA-MNPs and thus isolated effectively from the extract. The other two saponins detected in the extract (i.e. protodioscin and 26-O-β-D-glucopyranosyl-3β,20α,26-triol-25(R)-Δ(5,22)-dienofurostan-3-O-α-L-rhamnopyranosyl (1→2)-[α-L-rhamnopyranosyl (1→4)]-β-D-glucopyranoside) exhibited no affinity at all. Among the three saponins fished out, dioscin bound to HSA much stronger than gracillin and pseudo-protodioscin did. The results indicated that affinity interaction between HSA immobil...Continue Reading

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Citations

Sep 23, 2010·Pharmaceutical Research·Michał Piotr Marszałł
Jul 9, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liangliang LiuMijun Peng
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Aug 8, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Li YuanXun Liao
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Sep 21, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Songsong ZhangXiuhua Liu
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Jul 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qian-Long WangLin-Sen Qing
Jun 28, 2012·Analytical Methods : Advancing Methods and Applications·Lin-Sen QingXun Liao
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