Ligand-functionalized degradable polyplexes formed by cationic poly(aspartic acid)-grafted chitosan-cyclodextrin conjugates

Nanoscale
Hai-Qing SongFu-Jian Xu

Abstract

Polypeptide-based degradable polyplexes attracted considerable attention in drug delivery systems. Polysaccharides including cyclodextrin (CD), dextran, and chitosan (CS) were readily grafted with cationic poly(aspartic acid)s (PAsps). To further enhance the transfection performances of PAsp-based polyplexes, herein, different types of ligand (folic acid, FA)-functionalized degradable polyplexes were proposed based on the PAsp-grafted chitosan-cyclodextrin conjugate (CCPE), where multiple β-CDs were tied on a CS chain. The FA-functionalized CCPE (i.e., CCPE-FA) was obtained via a host-guest interaction between the CD units of CCPE and the adamantane (Ad) species of Ad-modified FA (Ad-FA). The resulting CCPE/pDNA, CCPE-FA/pDNA, and ternary CCPE-FA/CCPE/pDNA (prepared by layer-by-layer assembly) polyplexes were investigated in detail using different cell lines. The CCPE-based polyplexes displayed much higher transfection efficiencies than the CS-based polyplexes reported earlier by us. The ternary polyplexes of CCPE-FA/CCPE/pDNA produced excellent gene transfection abilities in the folate receptor (FR)-positive tumor cells. This work would provide a promising means to produce highly efficient polyplexes for future gene therapy ap...Continue Reading

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Citations

Mar 11, 2016·Molecular Biotechnology·Touba EslaminejadMehdi Ansari
Mar 8, 2016·ACS Applied Materials & Interfaces·Jing-Jun NieFujian Xu
Mar 29, 2016·Bioconjugate Chemistry·Jia LiuXulin Jiang
May 19, 2016·ACS Applied Materials & Interfaces·Jia LiuLin Wang
Oct 25, 2016·Chemical Society Reviews·Chunhua CaiLiquan Wang
Dec 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Peiqiong ZhouXiaoyan Yuan
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Zhenzhen ZhangXiang Zhou
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Prabhu Srinivas YavvariAasheesh Srivastava
Jan 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Raquel Mejia-ArizaJurriaan Huskens

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