Ligand rebinding: self-consistent mean-field theory and numerical simulations applied to surface plasmon resonance studies

European Biophysics Journal : EBJ
Manoj GopalakrishnanUwe C Täuber

Abstract

Rebinding of dissociated ligands from cell surface proteins can confound quantitative measurements of dissociation rates important for characterizing the affinity of binding interactions. This can be true also for in vitro techniques such as surface plasmon resonance (SPR). We present experimental results using SPR for the interaction of insulin-like growth factor-I (IGF-I) with one of its binding proteins, IGF binding protein-3 (IGFBP-3), and show that the dissociation, even with the addition of soluble heparin in the dissociation phase, does not exhibit the expected exponential decay characteristic of a 1:1 binding reaction. We thus consider the effect of (multiple) rebinding events and, within a self-consistent mean-field approximation, we derive the complete mathematical form for the fraction of bound ligands as a function of time. We show that, except for very low association rate and surface coverage, this function is nonexponential at all times, indicating that multiple rebinding events strongly influence dissociation even at early times. We compare the mean-field results with numerical simulations and find good agreement, although deviations are measurable in certain cases. Our analysis of the IGF-I-IGFBP-3 data indicat...Continue Reading

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Citations

Oct 11, 2008·The Journal of Biological Chemistry·Maria MitsiMatthew A Nugent
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Related Concepts

In Silico
Insulin-Like Growth Factor I
Plasma Protein Binding Capacity
Insulin-Like Growth Factor Binding Protein 3
Surface Plasmon Resonance
Protein Interaction Mapping

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