Ligand Size and Conformation Affect the Behavior of Nanoparticles Coated with in Vitro and in Vivo Protein Corona

ACS Applied Materials & Interfaces
Huajin ZhangHuile Gao

Abstract

Protein corona is immediately established on the surface of nanoparticles upon their introduction into biological milieu. Several studies have shown that the targeting efficiency of ligand-modified nanoparticles is attenuated or abolished owing to the protein adsorption. Here, transferrin receptor-targeting ligands, including LT7 (CHAIYPRH), DT7 (hrpyiahc, all d-form amino acids), and transferrin, were used to identify the influence of the ligand size and conformation on protein corona formation. The results showed that the targeting capacity of ligand-modified nanoparticles was lost after incubation with plasma in vitro, whereas it was partially retained after in vivo corona formation. Results from sodium dodecyl sulfate polyacrylamide gel electrophoresis and liquid chromatography-mass spectrometry revealed the difference in the composition of in vitro and in vivo corona, wherein the ligand size and conformation played a critical role. Differences were observed in cellular internalization and exocytosis profiles on the basis of the ligand and corona source.

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Citations

Nov 27, 2019·Nanotechnology·Francisca Villanueva-FloresLaura A Palomares
Mar 27, 2020·Pharmaceutics·Yanlong YinQian Yang
Jun 5, 2020·The AAPS Journal·Dongyu ChenMansoor Amiji
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Mar 11, 2020·Drug Delivery and Translational Research·Germán BerrecosoMaría José Alonso
Sep 28, 2018·Advanced Healthcare Materials·Xiaowen LiuZhuang Liu
Apr 22, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang ZhouHuile Gao
Oct 3, 2020·Biotechnology and Applied Biochemistry·Elisa Fasoli
Feb 16, 2021·ACS Applied Materials & Interfaces·Yadileiny PortillaDomingo F Barber
Apr 7, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yazhen WangHuile Gao
May 24, 2021·The Science of the Total Environment·Huiying GuoJinshao Ye
Sep 10, 2021·ACS Applied Materials & Interfaces·Dominyka DapkuteVitalijus Karabanovas
Oct 31, 2021·Comprehensive Reviews in Food Science and Food Safety·Guoxin CuiMingqian Tan

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