Ligand-specific sequential regulation of transcription factors for differentiation of MCF-7 cells.

BMC Genomics
Yuko SaekiMariko Okada-Hatakeyama

Abstract

Sharing a common ErbB/HER receptor signaling pathway, heregulin (HRG) induces differentiation of MCF-7 human breast cancer cells while epidermal growth factor (EGF) elicits proliferation. Although cell fates resulting from action of the aforementioned ligands completely different, the respective gene expression profiles in early transcription are qualitatively similar, suggesting that gene expression during late transcription, but not early transcription, may reflect ligand specificity. In this study, based on both the data from time-course quantitative real-time PCR on over 2,000 human transcription factors and microarray of all human genes, we identified a series of transcription factors which may control HRG-specific late transcription in MCF-7 cells. We predicted that four transcription factors including EGR4, FRA-1, FHL2, and DIPA should have responsibility of regulation in MCF-7 cell differentiation. Validation analysis suggested that one member of the activator protein 1 (AP-1) family, FOSL-1 (FRA-1 gene), appeared immediately following c-FOS expression, might be responsible for expression of transcription factor FHL2 through activation of the AP-1 complex. Furthermore, RNAi gene silencing of FOSL-1 and FHL2 resulted in ...Continue Reading

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References

Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H NishinaH Iba
Jun 15, 1988·Biochemical Pharmacology·A S AtallahP Hochstein
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K RyderD Nathans
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Milbrandt
Oct 1, 1988·Journal of Clinical and Experimental Neuropsychology·K M Adams
May 1, 1988·Molecular and Cellular Biology·D R Cohen, T Curran
Jan 1, 1993·The Biochemical Journal·V Vouret-CraviariJ Pouysségur
Dec 13, 1997·Biochimica Et Biophysica Acta·P F ZipfelC Skerka
Jan 11, 2000·Molecular and Cellular Biology·J E ReuschD J Klemm
Jun 8, 2002·Molecular and Cellular Biology·Latifa BakiriMoshe Yaniv
Jul 23, 2002·Nature Cell Biology·Leon O MurphyJohn Blenis
Mar 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Aurore Morlon, Paolo Sassone-Corsi
Jul 5, 2003·The Journal of Clinical Endocrinology and Metabolism·You-Hong Cheng, Stuart Handwerger
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew E Schade, Alan D Levine
Mar 23, 2005·BMC Bioinformatics·Alexey LarionovWilliam Miller
Sep 24, 2005·Cellular Signalling·Stephane Dedieu, Philippe Lefebvre
Nov 24, 2005·Current Biology : CB·Matthew Freeman

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Citations

Aug 2, 2012·Molecular and Cellular Biology·Berdymammet HojayevJoseph A Hill
Jan 15, 2014·PloS One·Naresh Doni Jayavelu, Nadav Bar
Jul 28, 2015·Gene·Cyanne Ye CaoStephen Kwok-Wing Tsui
May 9, 2015·Biophysical Journal·Sota TakanezawaYasushi Sako
May 5, 2017·Molecular Systems Biology·Florian UhlitzNils Blüthgen
Feb 13, 2015·The Journal of Pharmacology and Experimental Therapeutics·Aditya D JoshiCornelis J Elferink
Dec 31, 2015·Oncotarget·Michalis V KaramouzisAthanasios G Papavassiliou
Jul 2, 2020·International Journal of Molecular Sciences·Masa TsuchiyaKenichi Yoshikawa
Jan 17, 2021·Biophysical Journal·Jekabs KrigertsJekaterina Erenpreisa
Jun 30, 2021·Journal of Cancer Research and Clinical Oncology·Karen BräutigamFrank Köster
Nov 13, 2021·Molecular Pharmacology·Nikhil Y PatilAditya D Joshi

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Methods Mentioned

BETA
PCR
electrophoresis
chip
protein assay
Transfection
Assay
immunoprecipitation
co-immunoprecipitation assay
gene knockdown
immunoprecipitation assay

Software Mentioned

GATHER
Gene Ontology
GCOS
GeneChip
GeneChip Operating Software ( GCOS )

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