Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining

Proceedings of the National Academy of Sciences of the United States of America
Guangqing LuYu Zhang

Abstract

In eukaryotes, DNA double-strand breaks (DSBs), one of the most harmful types of DNA damage, are repaired by homologous repair (HR) and nonhomologous end-joining (NHEJ). Surprisingly, in cells deficient for core classic NHEJ factors such as DNA ligase IV (Lig4), substantial end-joining activities have been observed in various situations, suggesting the existence of alternative end-joining (A-EJ) activities. Several putative A-EJ factors have been proposed, although results are mostly controversial. By using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, we generated mouse CH12F3 cell lines in which, in addition to Lig4, either Lig1 or nuclear Lig3, representing the cells containing a single DNA ligase (Lig3 or Lig1, respectively) in their nucleus, was completely ablated. Surprisingly, we found that both Lig1- and Lig3-containing complexes could efficiently catalyze A-EJ for class switching recombination (CSR) in the IgH locus and chromosomal deletions between DSBs generated by CRISPR/Cas9 in cis-chromosomes. However, only deletion of nuclear Lig3, but not Lig1, could significantly reduce the interchromosomal translocations in Lig4(-/-) cells, suggesting the unique...Continue Reading

References

Feb 1, 1996·International Immunology·M NakamuraT Honjo
Jun 4, 2004·Molecular Cell·Josée Guirouilh-BarbatBernard S Lopez
Jun 4, 2008·Annual Review of Biochemistry·Tom Ellenberger, Alan E Tomkinson
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Cristian BoboilaBjoern Schwer
Apr 9, 2013·Frontiers in Genetics·Anabelle Decottignies
May 3, 2013·Cold Spring Harbor Perspectives in Biology·Kishore K ChiruvellaThomas E Wilson
May 21, 2013·DNA Repair·Teruaki Iyama, David M Wilson
Sep 21, 2013·Annual Review of Genetics·Ludovic Deriano, David B Roth
Mar 13, 2014·DNA Repair·Nicholas R PannunzioMichael R Lieber
Mar 13, 2014·DNA Repair·Philippe FritPatrick Calsou
Sep 24, 2015·The Biochemical Journal·Penny A Jeggo, Markus Löbrich
Oct 7, 2015·Trends in Cell Biology·Raphael CeccaldiAlan D D'Andrea

❮ Previous
Next ❯

Citations

Aug 4, 2016·Nucleic Acids Research·Brian R ShyBradley J Merrill
Sep 24, 2016·Genes·Samuel J BlackRichard T Pomerantz
Jun 1, 2017·Nature Communications·Ha Youn ShinLothar Hennighausen
Dec 7, 2016·Nature Communications·Raghavendra A ShamannaVilhelm A Bohr
Feb 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alexanda K LingAlberto Martin
Feb 9, 2018·International Journal of Molecular Sciences·Kez ClealDuncan Baird
Apr 21, 2018·The Journal of General Virology·Rutger David LuteijnEmmanuel J H J Wiertz
Jan 26, 2020·International Journal of Molecular Sciences·Karolina ŁuczkowskaBogusław Machaliński
May 5, 2020·Nucleic Acids Research·Adele Williamson, Hanna-Kirsti S Leiros
May 13, 2020·Briefings in Functional Genomics·Mohd AmirMd Imtaiyaz Hassan
Sep 1, 2020·The Journal of Experimental Medicine·Angela HelfrichtHaico van Attikum
Jul 9, 2017·Nature Communications·Alex N ZelenskyMarcel Tijsterman
Aug 10, 2018·Nature Reviews. Nephrology·Zachary WareJoncasStephen C Ekker
Dec 28, 2018·Nucleic Acids Research·Kate LiddiardDuncan M Baird
Jul 28, 2018·Frontiers in Cellular and Infection Microbiology·Elisa Azuara-LiceagaLuis G Brieba
Nov 6, 2018·The Journal of Clinical Investigation·Patrick MaffucciCharlotte Cunningham-Rundles
Oct 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jacob V LayerTovah A Day
Dec 10, 2017·Molecular Cancer Research : MCR·Franz Josef GassnerRoland Geisberger
Jul 22, 2019·DNA Repair·Keith W Caldecott
Oct 29, 2020·Trends in Cancer·Anna SchrempfJoanna I Loizou
Mar 11, 2021·Cell Reports·Gurushankar ChandramoulyRichard T Pomerantz
Apr 4, 2021·Cancers·Daniele CaraccioloPierfrancesco Tassone
Jun 10, 2021·Oncogene·Dale A Ramsden, Andre Nussenzweig
Jul 3, 2021·Biology·Marlo K ThompsonAishwarya Prakash
Nov 5, 2021·Cellular and Molecular Life Sciences : CMLS·Nagham Nafiz HindiDindial Ramotar
Dec 21, 2021·Frontiers in Cell and Developmental Biology·Xikui SunJunchao Dong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biophysics of CRISPR

This feed focuses on broad characteristics of the CRISPR system and the proteins associated with it.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.