PMID: 7542445Jul 1, 1995Paper

Ligation of the alpha 2-macroglobulin signalling receptor on macrophages induces protein phosphorylation and an increase in cytosolic pH

The Biochemical Journal
Uma K MisraS V Pizzo

Abstract

We have recently described an alpha 2-macroglobulin (alpha 2M) signalling receptor which is distinct from the low-density lipoprotein-related protein/alpha 2M receptor (LRP/alpha 2MR). Ligation of the macrophage signalling receptor by alpha 2M-methylamine stimulates production of several second messengers and involves a pertussis toxin-insensitive G-protein. We now report that binding of alpha 2M-methylamine, or the cloned M(r) = 20,000 receptor-binding fragment from rat alpha 1M, to macrophage alpha 2M signalling receptors induces protein phosphorylation. By use of a monoclonal antibody to phospholipase C gamma l (PLC gamma l) we were able to identify it as one target for protein phosphorylation. Phosphorylation was time and concentration dependent, being optimal at about 60 s of incubation and a 100-200 nM ligand concentration. By use of a second monoclonal antibody directed against phosphotyrosine, we were able to demonstrate that at least a portion of the label was incorporated into one or more tyrosine residues. PLC gamma l phosphorylation was then studied in membrane preparations at 4 degrees C in order to minimize serine or threonine modification. Preincubation of macrophage membranes with genistein, a tyrosine kinase in...Continue Reading

Citations

Jun 13, 1997·The Journal of Biological Chemistry·S G Rhee, Y S Bae
Jan 3, 1997·The Journal of Biological Chemistry·U K MisraS V Pizzo
Sep 3, 2005·Immunological Investigations·John G FacciponteJohn R Subjeck
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brent BerwinChristopher V Nicchitta
Jul 13, 2002·The Journal of Biological Chemistry·Uma Kant MisraSalvatore Vincent Pizzo
May 18, 1999·Biochemical and Biophysical Research Communications·E A HodsonJ S Lymn
May 23, 2001·Archives of Biochemistry and Biophysics·U K Misra, S V Pizzo

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