Light absorbing properties of indocyanine green (ICG) in solution and after adsorption to the retinal surface: an ex-vivo approach

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Christos HaritoglouAnselm Kampik

Abstract

To evaluate potential differences in light absorbing properties and stability of indocyanine green (ICG) adsorbed to the retinal surface and of ICG dissolved in water and balanced salt solution. The retina of four human donor eyes was prepared by removing the vitreous from the retinal surface. The inner surface of the specimen was covered with two to three drops of a 0.05% or 0.15% ICG solution respectively. After 1 min, the dye was removed by careful irrigation using BSS plus. The retinal specimens were then investigated by diffuse reflection spectroscopy (UV/VIS/NIR Spectrometer Lambda 900/Perkin Elmer equipped with a PELA-1020 integrating sphere accessory) and their absorption evaluated by the Kubelka-Munk function. To control the sensitivity of the setting, diffuse reflectance spectra of ICG adsorbed to a cellulose membrane and Al(2)O(3) were measured. For comparison, absorption spectra of ICG dissolved in water and BSS plus solution were measured in relation to ICG concentration and time using an UV/VIS/NIR Spectrometer Lambda 900/Perkin Elmer. On the retinal surface, absorption spectra exhibited a steep increase of absorption beginning at 620 nm, with a maximum at 736 nm (0.05%) and a shoulder at 745 (0.15%) and a second ...Continue Reading

References

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Dec 31, 2002·Investigative Ophthalmology & Visual Science·Arnd GandorferAnselm Kampik
May 27, 2003·Investigative Ophthalmology & Visual Science·Christos HaritoglouAnselm Kampik
Mar 23, 2004·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·I KrömerD Pauleikhoff
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Citations

Jul 13, 2006·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·C Haritoglou, A Kampik
Jan 10, 2009·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·H Langhals, C Haritoglou
Mar 8, 2012·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Christos HaritoglouAnselm Kampik
Jul 5, 2011·Journal of Biomedical Optics·Anna GeregaAdam Liebert
Nov 30, 2011·Photodiagnosis and Photodynamic Therapy·Hyun-Ju Lim, Chung-Hun Oh
Aug 18, 2009·Survey of Ophthalmology·Eduardo B RodriguesMichel E Farah
Jan 23, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Amir H TorbatiPatrick T Mather
Nov 28, 2009·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Srikant VaithilingamButrus T Khuri-Yakub
May 13, 2009·Acta Ophthalmologica·Paul B HenrichSiegfried Priglinger
Apr 29, 2011·Journal of Medicinal Chemistry·Heinz LanghalsChristos Haritoglou

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