Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods

Pharmaceutics
Takuya HashimotoKenji Kono

Abstract

Gene delivery to target cells is crucially important to establish gene therapy and regenerative medicine. Although various virus-based and synthetic molecule-based gene vectors have been developed to date, selective transfection in a site or a cell level is still challenging. For this study, both light-responsive and temperature-responsive synthetic gene vectors were designed for spatiotemporal control of a transfection system. 11-Mercaptoundecanoic acid-coated gold nanorods were mixed with polyamidoamine dendron-bearing lipids of two types having amino-terminus or ethoxydiethylene glycol-terminus to obtain hybrid vectors. Hybrid vectors were mixed further with pDNA. Then we investigated their physicochemical properties and transfection efficacy with or without near infrared laser irradiation. Hybrid vectors formed complexes with pDNA and exhibited enhanced photothermal property under near infrared laser irradiation compared with parent gold nanorods. Transfection efficacy of complexes was promoted considerably by brief laser irradiation soon after complex application to the cells. Analysis of intracellular distribution revealed that laser irradiation promoted the adsorption of complexes to the cells and cytosolic release of pD...Continue Reading

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Citations

Jul 3, 2021·Nanomaterials·Natalia Sánchez-ArribasAndrés Guerrero-Martínez

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Methods Mentioned

BETA
transfection
surface plasmon resonance
NMR
atomic force microscopy
transmission electron microscopy
Electrophoresis
flow cytometry
AFM

Software Mentioned

Prism
GraphPad
Science Lab 2003 Multi Gauge

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