PMID: 6315060Nov 30, 1983Paper

Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres

Biochimica Et Biophysica Acta
P R Rich, P Heathcote


(1) Purified bovine heart mitochondrial cytochrome b-c1 complex (ubiquinone-cytochrome c oxidoreductase) and photosynthetic reaction centres isolated from Rhodopseudomonas sphaeroides strain R-26 have been incorporated into lipid vesicles. In the presence of cytochrome c and ubiquinone-2, light activation caused a cyclic electron transfer involving both components. (2) Since cytochrome c is added outside the vesicles, it is both reduced by the cytochrome b-c1 complex and oxidised by the reaction centre on the outside of the vesicles. Ubiquinone-2, however, is reduced by the reaction centres at a site in contact with the inside of the vesicles, but the reduced form, ubiquinol-2, is oxidised by the cytochrome b-c1 complex at a site in contact with the outer aqueous phase. (3) In the presence of valinomycin plus K+, initiation of cyclic electron flow causes protons to move from inside the vesicles to the outer medium and the H +/2e- ratio was calculated to be close to 4.


Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N K PackhamP L Dutton
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M SchönfeldG Feher

Related Concepts

Ubiquinol 9
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Bos indicus
Lipid Bilayers
Mitochondria, Heart
Multienzyme Complexes
NADH, NADPH Oxidoreductases
Photic Stimulation
Plant Proteins

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