PMID: 9539726May 16, 1998Paper

Light-activated rhodopsin induces structural binding motif in G protein alpha subunit

Proceedings of the National Academy of Sciences of the United States of America
O G KisselevG R Marshall

Abstract

A large superfamily of transmembrane receptors control cellular responses to diverse extracellular signals by catalyzing activation of specific types of heterotrimeric GTP-binding proteins. How these receptors recognize and promote nucleotide exchange on G protein alpha subunits to initiate signal amplification is unknown. The three-dimensional structure of the transducin (Gt) alpha subunit C-terminal undecapeptide Gtalpha(340-350) IKENLKDCGLF was determined by transferred nuclear Overhauser effect spectroscopy while it was bound to photoexcited rhodopsin. Light activation of rhodopsin causes a dramatic shift from a disordered conformation of Gtalpha(340-350) to a binding motif with a helical turn followed by an open reverse turn centered at Gly-348, a helix-terminating C capping motif of an alphaL type. Docking of the NMR structure to the GDP-bound x-ray structure of Gt reveals that photoexcited rhodopsin promotes the formation of a continuous helix over residues 325-346 terminated by the C-terminal helical cap with a unique cluster of crucial hydrophobic side chains. A molecular mechanism by which activated receptors can control G proteins through reversible conformational changes at the receptor-G protein interface is demons...Continue Reading

References

Jun 17, 1988·Science·L G Presta, G D Rose
Jul 28, 1986·FEBS Letters·S K Burley, G A Petsko
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jun 17, 1988·Science·J S Richardson, D C Richardson
Jan 1, 1986·Photochemistry and Photobiology·R F BorkmanJ Dillon
May 21, 1974·Biochemistry·D S Papermaster, W J Dreyer
Jun 8, 1968·Nature·Y KitoY Sekoguti
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B K FungL Stryer
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A YamazakiM W Bitensky
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·O KisselevN Gautam
May 20, 1994·Science·R AuroraG D Rose
May 20, 1993·Nature·E A DratzH E Hamm
Dec 29, 1995·The Journal of Biological Chemistry·S Osawa, E R Weiss
Jan 5, 1996·The Journal of Biological Chemistry·E L MartinH E Hamm
Jan 25, 1996·Nature·D G LambrightP B Sigler
Aug 2, 1996·The Journal of Biological Chemistry·D A Toke, C E Martin
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Apr 1, 1996·Current Opinion in Cell Biology·H E Hamm, A Gilchrist
Oct 29, 1996·Biochimica Et Biophysica Acta·E J Helmreich, K P Hofmann
Mar 7, 1997·The Journal of Biological Chemistry·S AcharyaS S Karnik
Jan 1, 1997·Annual Review of Neuroscience·T GudermannG Schultz

❮ Previous
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Citations

Jan 5, 2002·Biopolymers·G R Marshall
Sep 6, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·Anna Maria DursiPaolo Rovero
Sep 7, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maren ClausRalf Paschke
May 13, 2005·Journal of Molecular Modeling·Jerzy CiarkowskiRafał Slusarz
Apr 28, 2011·Journal of Molecular Modeling·Won-joon SonSeokmin Shin
Jun 27, 2002·Trends in Pharmacological Sciences·Niki Zacharias, Dennis A Dougherty
Jan 9, 2003·Trends in Biochemical Sciences·Jacqueline Cherfils, Marc Chabre
Sep 19, 2003·Trends in Biochemical Sciences·Kevin D RidgeKrzysztof Palczewski
Apr 22, 2011·Journal of the American Chemical Society·Matthias ElgetiFranz J Bartl
Sep 1, 2012·Journal of the American Chemical Society·Xiange ZhengGarland R Marshall
Jul 26, 2013·Journal of the American Chemical Society·Matthias ElgetiMartin Heck
Aug 19, 2008·Journal of Medicinal Chemistry·Christina M TaylorGarland R Marshall
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Aug 8, 2006·Nature Structural & Molecular Biology·William M OldhamHeidi E Hamm
Oct 23, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·David L Farrens
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Patrick ScheererPeter W Hildebrand
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ned Van EpsWayne L Hubbell
Oct 5, 2012·The Journal of Biological Chemistry·Riad SeddikBernhard Bettler
May 1, 2013·Integrative and Comparative Biology·Yale J Passamaneck, Mark Q Martindale
Sep 18, 2007·Molecular Endocrinology·Krassimira AngelovaDavid Puett
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joseph A GoncalvesSteven O Smith
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bernhard KnierimWayne L Hubbell
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·O G KisselevK P Hofmann
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ExnerB Nürnberg
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·P RondardH R Bourne
Sep 11, 2014·Nature Communications·Michal SzczepekPatrick Scheerer
Dec 21, 2006·Chemical Biology & Drug Design·Matthew A AndersonGarland R Marshall
Jun 28, 2006·Photochemistry and Photobiology·Yoshinori Shichida, Takefumi Morizumi
Sep 19, 2006·Vision Research·K D RidgeN G Abdulaev
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Thomas P SakmarElias S Awad
Nov 26, 2015·PloS One·Alexander S RosePeter W Hildebrand
Nov 27, 2001·Biophysical Journal·R ArimotoG R Marshall

❮ Previous
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