Light-activated serotonin for exploring its action in biological systems

Chemistry & Biology
Adam C ReaTimothy M Dore

Abstract

Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.

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Citations

Jun 28, 2014·Developmental Biology·Martin BlumMichael V Danilchik
May 2, 2017·ACS Chemical Neuroscience·Ricardo CabreraRoberto Etchenique
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Aug 15, 2017·Journal of the American Chemical Society·Naeem AsadTimothy M Dore
Dec 7, 2019·The Journal of Organic Chemistry·Anna-Lisa K HennigTimothy M Dore
Sep 1, 2020·ACS Chemical Biology·Sangram GoreTimothy M Dore
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