PMID: 8585931Nov 1, 1995Paper

Light adaptation of bovine retinas in situ stimulates phosphatidylinositol synthesis in rod outer segments in vitro

Current Eye Research
A J Ghalayini, R E Anderson

Abstract

Light-stimulated phosphoinositide turnover has been reported in both vertebrate retina and isolated rod outer segments (ROS). In the current investigation, we examined the incorporation of [3H]-inositol in vitro in bovine ROS isolated from dark adapted (DROS) or bleached (BROS) retinas. Incorporation of [3H]-inositol into phosphoinositides in BROS was 3-5 fold higher than in DROS. The majority (approximately 90%) of [3H]-inositol was found in phosphatidylinositol (PI), whereas phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) accounted for 7-8% of the label. The enhanced labelling of PI was only observed when bovine retinas were light-adapted prior to ROS preparation, suggesting the requirement for an intact photoreceptor for the observed effect. Our data strongly suggest that bleaching of bovine retina in situ stimulates PI synthesis in isolated ROS in vitro.

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F HayashiA Yamazaki
Oct 1, 1991·Journal of Neurochemistry·A J GhalayiniR E Anderson
Jun 12, 1990·Biochemistry·B D Gehm, D G Mc Connell
Aug 1, 1990·Experimental Eye Research·H G ChoeR E Anderson
Apr 30, 1985·Biochemical and Biophysical Research Communications·F Hayashi, T Amakawa
Mar 1, 1985·Journal of Neurochemistry·R E AndersonJ G Hollyfield
Apr 1, 1969·Journal of Neurochemistry·J A Benjamins, B W Agranoff
Oct 30, 1984·Biochemical and Biophysical Research Communications·A Ghalayini, R E Anderson
Sep 24, 1981·Biochimica Et Biophysica Acta·R E Anderson, J G Hollyfield
Oct 1, 1993·The Biochemical Journal·I P UdovichenkoD J Takemoto
Aug 1, 1993·Current Eye Research·H H JungJ Pfeilschifter
Feb 1, 1955·Biochimica Et Biophysica Acta·L E HOKIN, M R HOKIN

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Citations

Sep 20, 2011·Molecular Neurobiology·Susan E Brockerhoff
Jul 30, 2009·Journal of Lipid Research·Raju V S Rajala
Oct 16, 2009·Journal of Lipid Research·Norma M GiustoMónica G Ilincheta de Boschero
Nov 10, 2015·Experimental Eye Research·Paola M NataliniNorma M Giusto
Jun 27, 2014·Scientific Reports·Raju V S RajalaRobert E Anderson
Jul 19, 2005·Gene·Suzanne L Inglis-BroadgateStephanie Halford
Oct 3, 2003·Current Biology : CB·Roger C Hardie
Jun 15, 2000·Journal of Neurochemistry·Z HuangR E Anderson
Dec 31, 2003·The Journal of Biological Chemistry·Feng HeTheodore G Wensel
Jun 11, 2020·Cells·Stella FinkelsteinEkaterina S Lobanova
Jun 18, 2020·Biology·Ammaji RajalaRaju V S Rajala
Jun 17, 2020·Journal of Lipid Research·Raju V S Rajala
Feb 9, 2021·Journal of Lipid Research·Raju V S Rajala

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