Light and electron microscope immunocytochemical localization of 5- and 12-lipoxygenases and cyclooxygenase enzymes in human granulosa cells from preovulatory follicles

Prostaglandins, Leukotrienes, and Essential Fatty Acids
D PridhamC L Cook

Abstract

Cellular and subcellular distribution of 5- and 12-lipoxygenases and cyclooxygenase enzymes were investigated in human granulosa cells from preovulatory follicles using light and electron microscope immunocytochemistry. The results demonstrated that all three enzymes are present in granulosa cells but not in minor contaminating red blood cells. While the distribution of cyclooxygenase and 12-lipoxygenase was relatively uniform among the granulosa cells, 5-lipoxygenase was not uniformly distributed among these cells. All three enzymes are present in microvillus plasma membranes, rough endoplasmic reticulum, cytoplasm, nuclear membranes and chromatin. In summary, 5- and 12-lipoxygenases and cyclooxygenase enzymes, which catalyze the transformation of arachidonic acid into different eicosanoids, are present in several subcellular organelles including nuclei of granulosa cells from preovulatory follicles.

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Citations

Sep 27, 2002·American Journal of Reproductive Immunology : AJRI·Fumihisa ChishimaMitsuo Honda
Apr 3, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S KurusuI Hashimoto
Jan 2, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Shiro KurusuMitsumori Kawaminami
Nov 30, 2018·Endocrine Reviews·Diane M DuffyThomas E Curry
Mar 25, 1996·The Journal of Comparative Neurology·C R ThoreD W Busija
Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto

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