PMID: 8605277Jan 1, 1996Paper

Light chain-restricted autoantibodies in chronic idiopathic thrombocytopenic purpura, but no evidence for circulating clone B-lymphocytes

Annals of Hematology
D StockelbergHans Wadenvik

Abstract

In chronic idiopathic thrombocytopenic purpura (ITP) platelet destruction is caused by antibodies directed against platelet membrane glycoproteins (GP), and the predominant autoantigens are known to be GPIb/IX and GPIIb/IIIa. In a recent study we reported that these antibodies frequently had a restricted light chain phenotype, thereby supporting a clonal origin. Similar findings and the presence of clonal B-cell populations in immune thrombocytopenias have been reported by others. In the present study we further explored the hypothesis of clonal B-cell expansions in chronic ITP. Twenty patients with chronic ITP were investigated. Antibodies were detected with an ELISA (MAIPA) specific for GPIb/IX and GPIIb/IIIa; circulating clonal B lymphocytes were assessed by flow-cytometric (FACS) clonal-excess analysis and by analyzing Ig-gene rearrangements (CDR3) with the PCR technique. Nine patients displayed a GP-specific antibody restricted to either kappa or lambda phenotype. However, FACS analysis and Ig-gene rearrangement studies did not disclose any circulating clonal B-cell population. Considering the sensitivity of the FACs analysis and Ig-gene rearrangement for detection of clonal B-cell populations, the hypothesis of clonally d...Continue Reading

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Citations

Sep 23, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·M J JacobinG Clofent-Sanchez
Jun 22, 2006·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Xiaoxia ChuDaoxin Ma
Aug 27, 2014·Journal of Neurology, Neurosurgery, and Psychiatry·Elisabeth A CatsBart C Jacobs
Jan 31, 2020·Frontiers in Oncology·Bruno Fattizzo, Wilma Barcellini
Sep 15, 1998·Acta Paediatrica. Supplement·H WadenvikM Hou
May 16, 2018·Frontiers in Immunology·Maurice SwinkelsA J Gerard Jansen

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