Light-controlled endosomal escape of the novel CD133-targeting immunotoxin AC133-saporin by photochemical internalization - A minimally invasive cancer stem cell-targeting strategy

Journal of Controlled Release : Official Journal of the Controlled Release Society
Monica BostadPål K Selbo

Abstract

The cancer stem cell (CSC) marker CD133 is an attractive target to improve antitumor therapy. We have used photochemical internalization (PCI) for the endosomal escape of the novel CD133-targeting immunotoxin AC133-saporin (PCIAC133-saporin). PCI employs an endocytic vesicle-localizing photosensitizer, which generates reactive oxygen species upon light-activation causing a rupture of the vesicle membranes and endosomal escape of entrapped drugs. Here we show that AC133-saporin co-localizes with the PCI-photosensitizer TPCS2a, which upon light exposure induces cytosolic release of AC133-saporin. PCI of picomolar levels of AC133-saporin in colorectal adenocarcinoma WiDr cells blocked cell proliferation and induced 100% inhibition of cell viability and colony forming ability at the highest light doses, whereas no cytotoxicity was obtained in the absence of light. Efficient PCI-based CD133-targeting was in addition demonstrated in the stem-cell-like, triple negative breast cancer cell line MDA-MB-231 and in the aggressive malignant melanoma cell line FEMX-1, whereas no enhanced targeting was obtained in the CD133-negative breast cancer cell line MCF-7. PCIAC133-saporin induced mainly necrosis and a minimal apoptotic response based ...Continue Reading

References

Sep 1, 1990·International Journal of Radiation Biology·K BergJ W Winkelman
May 1, 1988·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·K Berg, J Moan
Aug 24, 2000·International Journal of Cancer. Journal International Du Cancer·P K SelboK Berg
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·P K SelboK Berg
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Nancy L OleinickIrina Belichenko
Jan 7, 2004·Advanced Drug Delivery Reviews·Anders HøgsetKristian Berg
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Fiorenzo Stirpe
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Aug 12, 2006·The Journal of Pharmacology and Experimental Therapeutics·Pål K SelboKristian Berg
Feb 22, 2008·Current Pharmaceutical Biotechnology·Kristian BergAnders Høgset
May 12, 2009·International Journal of Cancer. Journal International Du Cancer·Stephanie L LomonacoChaya Brodie
Aug 25, 2009·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Claudia DittfeldLeoni A Kunz-Schughart
Oct 27, 2009·Autophagy·John J ReinersDavid Kessel
Nov 26, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Josephine WoodhamsStephen G Bown
Jul 6, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pål Kristian SelboKristian Berg
Jul 16, 2010·Journal of Cellular and Molecular Medicine·Michael DewaelePatrizia Agostinis
Aug 3, 2010·Journal of Immunological Methods·Suresh Kumar SwaminathanJohn R Ohlfest
May 28, 2011·CA: a Cancer Journal for Clinicians·Patrizia AgostinisJakub Golab
Nov 8, 2011·Lasers in Surgery and Medicine·Anette WeyergangKristian Berg
Feb 22, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pål Kristian SelboKristian Berg
Apr 10, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Monica BostadPål K Selbo
Jun 1, 2013·Gynecologic Oncology·Amy P N SkubitzDaniel A Vallera
Jul 23, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Suresh Kumar SwaminathanJayanth Panyam
Oct 10, 2013·Toxins·Letizia PolitoAndrea Bolognesi

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Citations

Aug 6, 2015·Nanomedicine·Vaibhav MundraRam I Mahato
May 12, 2016·Bioorganic & Medicinal Chemistry Letters·Kazunori WatanabeTakashi Ohtsuki
Feb 14, 2018·Toxins·Francesco GiansantiRodolfo Ippoliti
Feb 25, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Sai Kiran S S PindiproluPavan Kumar Chintamaneni
Aug 23, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Sai Kiran S S PindiproluVeera Venkata Satyanarayana Reddy Karri
Nov 27, 2015·Breast Cancer : Basic and Clinical Research·Valéria B de Souza, André A Schenka
Sep 22, 2018·Advanced Healthcare Materials·Liang ChengZhiyuan Zhong
Apr 25, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Menghuan LiKaiyong Cai
Oct 4, 2019·Cell Biology International·Morteza AkbariBehzad Baradaran
Jan 31, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Kaicheng Liang, Hangrong Chen
Mar 23, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M S EngP K Selbo
Dec 7, 2019·Current Stem Cell Research & Therapy·Plabon K DasFarhadul Islam
Jul 3, 2019·Seminars in Cancer Biology·Monica MarzagalliPatrizia Limonta
Mar 12, 2021·Advanced Healthcare Materials·Riku KawasakiKazunari Akiyoshi
Apr 16, 2020·Anti-cancer Agents in Medicinal Chemistry·Nkune W NkuneHeidi Abrahamse

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