Light-Dependent OCT Structure Changes in Photoreceptor Degenerative rd 10 Mouse Retina

Investigative Ophthalmology & Visual Science
Yichao LiHaohua Qian

Abstract

Using optical coherence tomography (OCT) to analyze the effects of light/dark adaptation in a mouse model of inherited photoreceptor degeneration (rd10), and to study dynamics of subretinal fluid during the progress of retinal degeneration. rd10 and wild-type (WT) C57BL/6J mice were reared in cyclic light or darkness and imaged with Bioptigen UHR-OCT or Spectralis HRA+OCT after adaptation to either light or darkness. OCT images from rd10 mice were analyzed at three progressive stages of degeneration. After light-adaptation, stage I (postnatal age [P]26-29) eyes demonstrated no apparent subretinal fluid. At stage II (P32-38), subretinal fluid was present and restricted to parapapillary area, while at stage III (P44-45) extensive subretinal fluid was present across many retinal areas. Following overnight dark-adaptation, WT eyes showed a large reduction in outer retinal thickness (4.6 ± 1.4 μm, n = 16), whereas this change was significantly smaller in stage I rd10 eyes (1.5 ± 0.5 μm, n = 14). In stage II rd10 eyes, dark-adaptation significantly reduced the extent of subretinal fluid, with the amount of reduction correlating with the amount of fluid pre-existing in the light-adapted state. However, dark-adaptation did not signific...Continue Reading

Citations

Nov 20, 2018·Investigative Ophthalmology & Visual Science·Michael T MassengillAlfred S Lewin
Dec 15, 2018·Investigative Ophthalmology & Visual Science·Bruce A BerkowitzRobin Roberts
Apr 18, 2019·Investigative Ophthalmology & Visual Science·Bruce A BerkowitzHaohua Qian
May 23, 2019·Frontiers in Neuroscience·Viviana Rincón MontesAndreas Offenhäusser
Aug 25, 2020·Translational Vision Science & Technology·Ying V LiuMandeep S Singh
Apr 2, 2020·Investigative Ophthalmology & Visual Science·Johnny Di PierdomenicoDiego García-Ayuso
Oct 14, 2020·Scientific Reports·Michael T MassengillAlfred S Lewin
Nov 18, 2020·Scientific Reports·V Rincón MontesA Offenhäusser
Mar 7, 2021·International Journal of Molecular Sciences·Maximilian WeissStephanie C Joachim
Oct 27, 2020·Frontiers in Cellular Neuroscience·Yichao LiHaohua Qian
Nov 7, 2020·Investigative Ophthalmology & Visual Science·Bruce A BerkowitzRobin Roberts

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