Light-dependent subcellular translocation of Gqalpha in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINAC

Journal of Cell Science
Michelle A CroninSusan Tsunoda

Abstract

We examine the light-dependent subcellular translocation of the visual G(q)alpha protein between the signaling compartment, the rhabdomere and the cell body in Drosophila photoreceptors. We characterize the translocation of G(q)alpha and provide the first evidence implicating the involvement of the photoreceptor-specific myosin III NINAC in G(q)alpha transport. Translocation of G(q)alpha from the rhabdomere to the cell body is rapid, taking less than 5 minutes. Higher light intensities increased the quantity of G(q)alpha translocated out of the rhabdomeres from 20% to 75%, consistent with a mechanism for light adaptation. We demonstrate that translocation of G(q)alpha requires rhodopsin, but none of the known downstream phototransduction components, suggesting that the signaling pathway triggering translocation occurs upstream of G(q)alpha. Finally, we show that ninaC mutants display a significantly reduced rate of G(q)alpha transport from the cell body to the rhabdomere, suggesting that NINAC might function as a light-dependent plus-end motor involved in the transport of G(q)alpha.

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Citations

Jun 14, 2005·Pflügers Archiv : European journal of physiology·Craig Montell
May 10, 2007·Pflügers Archiv : European journal of physiology·Tao Wang, Craig Montell
Mar 21, 2007·Biochemistry·Karen KemplerBarbara-Anne Battelle
Jun 15, 2007·Biochemistry·Yannick MarrariPhilip B Wedegaertner
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Rebecca ElsaesserCraig Montell
Sep 10, 2010·The Journal of Biological Chemistry·Omar A QuinteroChristopher M Yengo
Mar 4, 2009·The Journal of Biological Chemistry·Haiqin LuBih-Hwa Shieh
May 25, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shahar FrechterBaruch Minke
Dec 17, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lina NiHong-Sheng Li
Nov 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Paloma T Gonzalez-BellidoMikko Juusola
Jun 18, 2005·The Journal of Physiology·Craig Montell
Apr 14, 2012·Trends in Neurosciences·Craig Montell
Feb 7, 2012·The European Journal of Neuroscience·Matthew J Van HookDavid M Berson
Aug 11, 2007·Molecular and Cellular Neurosciences·Parthena D SanxaridisSusan Tsunoda
Jun 20, 2012·Acta Biochimica Et Biophysica Sinica·Zdenka Drastichova, Jiri Novotny
Jul 19, 2005·Physiology·Mira Krendel, Mark S Mooseker
Oct 3, 2006·The Journal of Biological Chemistry·Taketoshi KambaraMitsuo Ikebe
Nov 1, 2006·The Journal of Biological Chemistry·Andréa C DoséChristopher M Yengo
Jul 15, 2020·Proteomics·Bharath Kumar RaghuramanAndrej Shevchenko
Jan 31, 2008·Biochemistry·Andréa C DoséChristopher M Yengo
Feb 9, 2013·The Journal of Experimental Biology·Barbara-Anne BattelleCristina D Gaddie
Jun 22, 2006·Journal of Cell Science·Michelle A CroninSusan Tsunoda

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