Light-Directed Synthesis of High-Density Peptide Nucleic Acid Microarrays

ACS Combinatorial Science
Feipeng YangZheng-Chun Liu

Abstract

Peptide nucleic acids (PNAs) are a class of nucleic acid mimics that can bind to the complementary DNA or RNA with high specificity and sensitivity. PNA-based microarrays have distinct characteristics and have improved performance in many aspects compared to DNA microarrays. A new set of PNA monomers has been synthesized and used as the building blocks for the preparation of high density PNA microarrays. These monomers have their backbones protected by the photolabile group 2-(2-nitrophenyl)propyloxy carbonyl (NPPOC), and their exocyclic amino groups protected by amide carbonyl groups. A light-directed synthesis system was designed and applied to the in situ synthesis of a PNA microarray with a density of over 10,000 probes per square centimeter. This PNA microarray was able to detect single and multiple base-mismatches correctly with a high discrimination ratio.

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Citations

Apr 25, 2018·Chemical Communications : Chem Comm·P K Kuroishi, A P Dove
Sep 7, 2018·Angewandte Chemie·Jory LietardMark M Somoza
Aug 25, 2020·Engineering Reports : Open Access·Kshitij Rb SinghRavindra Pratap Singh
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Apr 27, 2019·Biosensors & Bioelectronics·Bo DongZhengchun Liu
Jan 10, 2017·ACS Sustainable Chemistry & Engineering·K HölzM M Somoza
Jul 6, 2019·ACS Combinatorial Science·Matthew T Holden, Lloyd M Smith

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