Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium

Proceedings of the National Academy of Sciences of the United States of America
Kathryn R FixenCaroline S Harwood

Abstract

Nitrogenase is an ATP-requiring enzyme capable of carrying out multielectron reductions of inert molecules. A purified remodeled nitrogenase containing two amino acid substitutions near the site of its FeMo cofactor was recently described as having the capacity to reduce carbon dioxide (CO2) to methane (CH4). Here, we developed the anoxygenic phototroph, Rhodopseudomonas palustris, as a biocatalyst capable of light-driven CO2 reduction to CH4 in vivo using this remodeled nitrogenase. Conversion of CO2 to CH4 by R. palustris required constitutive expression of nitrogenase, which was achieved by using a variant of the transcription factor NifA that is able to activate expression of nitrogenase under all growth conditions. Also, light was required for generation of ATP by cyclic photophosphorylation. CH4 production by R. palustris could be controlled by manipulating the distribution of electrons and energy available to nitrogenase. This work shows the feasibility of using microbes to generate hydrocarbons from CO2 in one enzymatic step using light energy.

References

Mar 13, 2017·Photosynthesis Research·Silas Busck MellorPoul Erik Jensen
May 1, 2018·Environmental Microbiology·Kathryn R FixenCaroline S Harwood
Jan 18, 2018·Nature Microbiology·Yanning ZhengCaroline S Harwood
Mar 3, 2019·Applied and Environmental Microbiology·Yanning Zheng, Caroline S Harwood
Jul 28, 2020·Applied and Environmental Microbiology·Katja E LuxemXinning Zhang
Dec 28, 2019·Applied Microbiology and Biotechnology·Brett M Barney
Jul 14, 2017·Biotechnology for Biofuels·Devin F R DoudLargus T Angenent
Mar 30, 2020·Applied and Environmental Microbiology·Zhaobao WangJianming Yang

Citations

Jul 21, 2004·Nature Reviews. Microbiology·Ray Dixon, Daniel Kahn
Oct 7, 2004·The Journal of Biological Chemistry·Brett M BarneyLance C Seefeldt
Jan 16, 2007·Applied and Environmental Microbiology·Federico E ReyCaroline S Harwood
Apr 2, 2010·Environmental Science & Technology·Jeffrey M FoleyKorneel Rabaey
Jun 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·James B McKinlay, Caroline S Harwood
Oct 5, 2010·Applied and Environmental Microbiology·Jean J HuangCaroline S Harwood
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhi-Yong YangLance C Seefeldt
Apr 20, 2013·Biochimica Et Biophysica Acta·Lance C SeefeldtDennis R Dean
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Related Concepts

In Vivo
Photophosphorylation
Organic acids, Gynecological
Alcohols
Bacterial Proteins
Abnormal Fragmented Structure
Gentamicins
Methane
Phosphocreatine
Organic Chemicals

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