Light-Driven Nano-oscillators for Label-Free Single-Molecule Monitoring of MicroRNA

Nano Letters
Zixuan ChenJun-Jie Zhu

Abstract

Here, we present a mapping tool based on individual light-driven nano-oscillators for label-free single-molecule monitoring of microRNA. This design uses microRNA as a single-molecule damper for nano-oscillators by forming a rigid dual-strand structure in the gap between nano-oscillators and the immobilized surface. The ultrasensitive detection is attributed to comparable dimensions of the gap and microRNA. A developed surface plasmon-coupled scattering imaging technology enables us to directly measure the real-time gap distance vibration of multiple nano-oscillators with high accuracy and fast dynamics. High-level and low-level states of the oscillation amplitude indicate melting and hybridization statuses of microRNA. Lifetimes of two states reveal that the hybridization rate of microRNA is determined by the three-dimensional diffusion. This imaging technique contributes application potentials in a single-molecule detection and nanomechanics study.

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Citations

Sep 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xuemei YinJinghua Yu
Oct 31, 2018·Biosensors·Marta Maria Pereira da Silva Neves, Daniel Martín-Yerga
Dec 2, 2020·Small·Rachel E ArmstrongPeter Zijlstra
Aug 14, 2019·Biosensors & Bioelectronics·Hiroshi AokiBrandon Matthews
Feb 24, 2021·Analytical Chemistry·Peng Fei GaoCheng Zhi Huang
May 3, 2021·Nano Convergence·Despina P Kalogianni
May 6, 2020·Analytical Chemistry·Nicole Cathcart, Jennifer I L Chen
Oct 8, 2019·Analytical Chemistry·Xian ChenHuanghao Yang
Sep 30, 2021·Chemical Reviews·Lee PriestPhilipp Kukura

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