Light element analysis of individual bacteria by x-ray microanalysis.

Applied and Environmental Microbiology
S NorlandM Heldal

Abstract

A method based on X-ray microanalysis (XRMA) with the transmission electron microscope for measurement of total amounts of elements in single microbial cells has been developed. All major elements in cells except hydrogen can be measured simultaneously. XRMA provided N/C ratios (means (plusmn) standard errors of the mean) for stationary-phase and growing Escherichia coli of 0.23 (plusmn) 0.01 and 0.30 (plusmn) 0.01, respectively, while CHN analysis gave values of 0.276 and 0.307, respectively, for samples from the same cultures. Analyses of free coccoliths from Emiliana huxleyi provided weight fractions close to those of CaCO(inf3): 0.35 (plusmn) 0.01, 0.15 (plusmn) 0.01, and 0.47 (plusmn) 0.01 for calcium, carbon, and oxygen, respectively. Calibration is based on monodisperse latex beads and on microdrops of defined compounds. Elements in particles in the size range from 5 fg to 500 pg are measured with a relative precision between 500 and 5,000 ppm, depending on size. As a single-cell method, XRMA avoids the shortcomings of commonly used fractionation techniques associated with bulk methods, which are based on centrifugation or filtration. On the basis of morphology and XRMA, particles may be classified more precisely into gr...Continue Reading

References

Apr 1, 1977·Applied and Environmental Microbiology·S W WatsonF W Valois
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Feb 1, 1990·Applied and Environmental Microbiology·K Y BørsheimM Heldal
Nov 1, 1985·Applied and Environmental Microbiology·M HeldalO Tumyr
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Jun 1, 1987·Applied and Environmental Microbiology·S Lee, J A Fuhrman
Mar 1, 1987·Microbial Ecology·S NorlandO Tumyr

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Citations

May 15, 2013·Environmental Microbiology·Carolina GrobDavid J Scanlan
Sep 23, 2011·The ISME Journal·Mikal HeldalGunnar Bratbak
Dec 12, 2012·Environmental Microbiology·A K SteenberghH J Laanbroek
Jun 1, 2002·Applied and Environmental Microbiology·Katarina VredeGunnar Bratbak
May 23, 2015·FEMS Microbiology Reviews·Mark A LeverBo Barker Jørgensen
Aug 26, 2018·Nature Microbiology·Ed K HallMatthew D Wallenstein

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