Light enhanced bone regeneration in an athymic nude mouse implanted with mesenchymal stem cells embedded in PLGA microspheres

Biomaterials Research
Ji Sun Park, Keun-Hong Park

Abstract

Biodegradable microspheres fabricated from poly (Lactic-co-glycolic acid) (PLGA) have attracted considerable attention in the bone tissue regeneration field. In this study, rabbit mesenchymal stem cells (rMSCs) adherent to PLGA microspheres were implanted into athymic nude mice and irradiated with 647 nm red light to promote bone formation. It was found that irradiating rMSCs with high levels of red light (647 nm) from an LED (light-emitting diode) increased levels of bone specific markers in rMSCs embedded on PLGA microspheres. These increased expressions were observed by RT-PCR, real time-QPCR, immunohistochemistry (IHC), and von Kossa and Alizarin red S staining. Microsphere matrices coated with rMSCs were injected into athymic nude mice and irradiated with red light for 60 seconds showed significantly greater bone-specific phenotypes after 4 weeks in vivo. The devised PLGA microsphere matrix containing rMSCs and irradiation with red light at 647 nm process shows promise as a means of coating implantable biomedical devices to improve their biocompatibilities and in vivo performances.

References

Feb 28, 1992·Journal of Photochemistry and Photobiology. B, Biology·R LubartI Laulicht
Oct 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·H FriedmannS Rochkind
Mar 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·V ManteifelT Karu
Feb 12, 1998·Journal of Photochemistry and Photobiology. B, Biology·M SchafferE Dühmke
Jan 1, 1997·Journal of Clinical Laser Medicine & Surgery·F A Al-Watban, X Y Zhang
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·T Karu
Dec 31, 2003·Biochemical and Biophysical Research Communications·Takehiro MatsubaraYukio Kato
Jul 23, 2005·Lasers in Surgery and Medicine·Kimberly R ByrnesJuanita J Anders
Aug 19, 2005·Journal of Photochemistry and Photobiology. B, Biology·Albert AmatJuanita J Anders
Nov 17, 2006·Biochemical and Biophysical Research Communications·I-Chi LeeTai-Horng Young
Jan 17, 2007·Journal of Photochemistry and Photobiology. B, Biology·Gopalendu PalDarrell B Tata
May 4, 2007·Journal of Photochemistry and Photobiology. B, Biology·Sandra B RezendeEdison P Maldonado
May 25, 2007·Biochemical and Biophysical Research Communications·Warren L GraysonTeng Ma
Oct 2, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ji Sun ParkKeun-Hong Park
Apr 4, 2008·Lasers in Medical Science·Hyung Keun KimTaek Rim Yoon

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB009345
AY633663

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.