Light-harvesting superstructures of green plant chloroplasts lacking photosystems

Plant, Cell & Environment
Erica BelgioAlexander V Ruban

Abstract

The light-harvesting antenna of higher plant photosystem II (LHCII) is the major photosynthetic membrane component encoded by an entire family of homologous nuclear genes. On the contrary, the great majority of proteins of photosystems and electron transport components are encoded by the chloroplast genome. In this work, we succeeded in gradually inhibiting the expression of the chloroplast genes that led to the disappearance of the photosystem complexes, mimicking almost total photoinhibition. The treated plants, despite displaying only some early signs of senescence, sustained their metabolism and growth for several weeks. The only major remaining membrane component was LHCII antenna that formed superstructures - stacks of dozens of thylakoids or supergrana. Freeze-fracture electron microscopy revealed specific organization, directly displaying frequently bifurcated membranes with reduced or totally absent photosystem II (PSII) reaction centre complexes. Our findings show that it is possible to accumulate large amounts of light-harvesting membranes, organized into three-dimensional structures, in the absence of reaction centre complexes. This points to the reciprocal role of LHCII and PSII in self-assembly of the three-dimens...Continue Reading

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Citations

Aug 4, 2015·Journal of Photochemistry and Photobiology. B, Biology·Maxwell A WareAlexander V Ruban
Dec 7, 2017·Plant, Cell & Environment·Alexandra J TownsendAlexander V Ruban
Jan 1, 2015·Nature Plants·Alexander V Ruban, Matthew P Johnson
Nov 30, 2018·The Plant Journal : for Cell and Molecular Biology·Yugo Lima-MeloEva-Mari Aro
Mar 10, 2020·Journal of Experimental Botany·Francesco SacconAlexander V Ruban
Sep 1, 2017·Scientific Reports·Pascal AlbaneseCristina Pagliano
May 26, 2018·FEBS Letters·Alexander V Ruban
Oct 28, 2019·The Biochemical Journal·Petar H Lambrev, Parveen Akhtar
Apr 14, 2017·Photosynthesis Research·Erica BelgioRadek Kaňa
Apr 4, 2021·International Journal of Molecular Sciences·Aurélie CrepinRadek Kaňa

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