Light-Induced Activation of c-Met Signalling by Photocontrolled DNA Assembly

Chemistry : a European Journal
Shan ChenHuang-Hao Yang

Abstract

Optical manipulation appears to be a powerful tool for spatiotemporally controlling a variety of cellular functions. Herein, a photocontrolled DNA assembly approach is described which enables light-induced activation of cellular signal transduction by triggering protein dimerization (c-Met signalling in this case). Three kinds of DNA probes are designed, including a pair of receptor recognition probes with adaptors and a blocker probe with a photocleavable linker (PC-linker). By implementing PC-linkers in blocker probes, the designed DNA probes response to light irradiation, which then induces the assembly of receptor recognition probes through adaptor complementing. Consequently, light-mediated DNA assembly promotes the dimerization of c-Met receptors, resulting in activation of c-Met signalling. It is demonstrated that the proposed photocontrolled DNA assembly approach is effective for regulating c-Met signalling and modulating cellular behaviours, such as cell proliferation and migration. Therefore, this simple approach may offer a promising strategy to manipulate cell signalling pathways precisely in living cells.

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Citations

Feb 13, 2019·Chemical Communications : Chem Comm·Ryosuke UekiShinsuke Sando
Aug 1, 2019·Chembiochem : a European Journal of Chemical Biology·Jiahui FanZhou Nie
Feb 11, 2020·Chemical Society Reviews·Jingying LiHuanghao Yang
Sep 2, 2020·Chemical Society Reviews·Sean B Yeldell, Oliver Seitz
Nov 20, 2020·Chemical Science·Anna V Leopold, Vladislav V Verkhusha
May 25, 2021·Chemical Communications : Chem Comm·Ryosuke UekiShinsuke Sando
Jun 19, 2021·Angewandte Chemie·Momoko AkiyamaShinsuke Sando
Sep 9, 2021·Biomaterials Science·Qunye HeZhenbao Liu
Oct 5, 2021·Chemical Society Reviews·Lanlan ChenHuanghao Yang
Oct 20, 2021·Chembiochem : a European Journal of Chemical Biology·Soumya SethiMasayuki Endo

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