PMID: 6108215Oct 1, 1980Paper

Light-induced appearance of polysomal poly(A)-rich messenger RNA during greening of barley plants

European Journal of Biochemistry
H HeinzeM Kiper

Abstract

Changes in polysomal poly(A)-rich mRNA during greening of etiolated barley plants were studied by the technique of cDNA-mRNa hybridization. Hybridizaiton data of the homologous reactions reveal that in etiolated as well as in greened shoots a complexity of 5 X 10(7) nucleotides or about 33000 different average-sized mRNAs are present. These are organized in different abundancy classes with 94% of the total complexicity present in each of the slowest reacting class representing rare messengers. Heterologous hybridizations indicate that 92% of all polysomal poly(A)-rich mRNAs in etiolated shoots are complementary to those of greened and 82% of 'green' poly(A)-rich mRNAs are complementary to white ones. It is shown that the abundant mRNA clases are essentially responsible for these differences. The prevalent classes making up 15% ('white') and 31% ('green') of the poly(A)-rich mRNA mass but comprising only a complexity of 1.8 X 10(4) and 2.1 X 10(4) nucleotides are identical to 50% with each other. Hybridization of isolated prevalent 'green' cDNA with whole 'white' poly(A)-rich mRNA indicates that the additionally appearing 50% prevalent green messengers must be regarded as green-specific, only present in polysomal poly(A)-rich mR...Continue Reading

References

Oct 1, 1977·Nucleic Acids Research·E Y Friedman, M Rosbash
May 5, 1978·Science·V B ReddyS M Weissman
Jan 1, 1979·Nucleic Acids Research·M KiperG Richter
Jun 8, 1979·Science·E H Davidson, R J Britten
May 1, 1978·Nucleic Acids Research·T J QuinlanM J Getz
Jun 1, 1977·Nucleic Acids Research·W R PearsonR J Britten
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M J SmithE H Davidson
Jul 19, 1974·Nature·J O BishopM Richardson
Apr 1, 1980·Cell·J C Kamalay, R B Goldberg

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