PMID: 375978Apr 19, 1979Paper

Light-induced calcium release in isolated intact cattle rod outer segments upon photoexcitation of rhodopsin

Biochimica Et Biophysica Acta
U B KauppW Junge

Abstract

By applying flash-spectrophotometry with the calcium-indicating dye arsenazo III rapid light-triggered calcium release in various cattle rod outer segment preparations was studied. It is shown that light-induced calcium signals can be unambiguously discriminated from underlying absorption changes due to photolysis of rhodopsin and apparent absorption changes resulting from lightscattering transients. The following results have been obtained: 1. Calcium-induced arsenazo III responses can be quantitatively and kinetically resolved within the time domain of the visual transduction process. 2. Photoexcitation of rhodopsin results in calcium release from intradiscal binding sites. 3. Calcium released does not appear in the cytoplasmic space unless the disc membrane is made permeable to calcium ions by an ionophore. 4. The shortest observed half-rise time of calcium release (300 ms) is possibly limited by the ionophore. 5. The stoichiometric ratio of calcium released/rhodopsin bleached is 0.5 at a free calcium concentration of 2 microM. The amount of calcium released is proportional to the precentage of rhodopsin bleaching (from 1--10%). 6. Upon disruption of the disc stack by lysis of intact rod outer segments the light-induced calc...Continue Reading

References

Apr 19, 1979·Biochimica Et Biophysica Acta·P P SchnetkampF J Daemen
Mar 25, 1975·Biochimica Et Biophysica Acta·M Chabre, A Cavaggioni
Jul 18, 1975·Biochimica Et Biophysica Acta·R T Sorbi, A Cavaggioni
Sep 1, 1975·Acta Physiologica Scandinavica·K Hemminki
Sep 1, 1971·The Journal of General Physiology·D BowndsW Robinson
Feb 22, 1974·Nature·W T MasonE W Abrahamson

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Citations

Mar 20, 1980·Molecular and Cellular Biochemistry·M WellerP Mandel
Mar 22, 1985·Neuroscience Letters·R Salceda
Apr 1, 1981·The Journal of General Physiology·S McLaughlin, J Brown
Feb 1, 1987·The Journal of General Physiology·G D NicolM D Bownds
Jun 1, 1980·Photochemistry and Photobiology·H Shichi, C N Rafferty
Apr 19, 1979·Biochimica Et Biophysica Acta·P P SchnetkampF J Daemen
Mar 16, 1982·Biochemistry·P N TyminskiD F O'Brien
Jan 29, 1985·Biochemistry·P P Schnetkamp, U B Kaupp
Oct 1, 1980·Photochemistry and Photobiology·D S Cafiso, W L Hubbell

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