Light induced cytotoxicity of nitrofurantoin toward murine melanoma

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Lucimara P FerreiraRoberto Santana da Silva

Abstract

The cytotoxicity of nitrofurantoin (NFT) in the dark and after light exposure (UVA irradiation, λ = 385 nm) was evaluated in murine melanoma B16F10 cells. NFT induces both cell proliferation and inhibition of cell viability. The dominance of one or the other effect depends on the drug concentration, incubation time (tinc) and irradiation dose. The uptake of NFT in these cells, as well as its photocytotoxicity, reaches saturation after 24 hours of incubation. The mechanism of cell death in the dark is associated with the enzymatic release of nitric oxide (NO). The increase of NFT cytotoxicity under light irradiation is associated with the increase of NO concentration due to photorelease. NO photorelease by NFT in solution was confirmed by chemiluminescence, while NO formation in cells was confirmed by fluorescence microscopy using DAF-2DA, a specific indicator of NO in living cells. The NFT does not enter nuclei, distributing preferentially in the cell cytoplasm, as shown by fluorescence microscopy.

References

Dec 28, 1992·Science·E Culotta, D E Koshland
Aug 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·G M Beijersbergen van Henegouwen
Nov 1, 1988·Photochemistry and Photobiology·R W BuskerW Vasbinder
Dec 1, 1982·Biochemical Pharmacology·R J YoungmanE F Elstner
Jun 6, 2000·British Journal of Cancer·M KorbelikD J Chaplin
Apr 11, 2002·Chemical Reviews·Tetsuo Nagano, Tetsuhiko Yoshimura
Nov 24, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N A DaghastanliI E Borissevitch
Sep 27, 2006·Environmental Science & Technology·Betsy L EdhlundKristopher McNeill
Apr 29, 2008·Free Radical Biology & Medicine·Douglas D ThomasDavid A Wink
Jun 15, 2011·Free Radical Biology & Medicine·Carla S OliveiraMauricio S Baptista
May 31, 2012·Chemical Biology & Drug Design·Niroshini M GilesGregory I Giles
Oct 12, 2016·Investigational New Drugs·Valentina RapozziLuigi E Xodo

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Citations

Mar 7, 2021·Biomolecules·Allison M RiceS Bruce King

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