Light-induced disulfide dimerization of recoverin under ex vivo and in vivo conditions

Free Radical Biology & Medicine
Evgeni Yu ZerniiSergei E Permyakov

Abstract

Despite vast knowledge of the molecular mechanisms underlying photochemical damage of photoreceptors, linked to progression of age-related macular degeneration, information on specific protein targets of the light-induced oxidative stress is scarce. Here, we demonstrate that prolonged intense illumination (halogen bulb, 1500 lx, 1-5 h) of mammalian eyes under ex vivo (cow) or in vivo (rabbit) conditions induces disulfide dimerization of recoverin, a Ca(2+)-dependent inhibitor of rhodopsin kinase. Western blotting and mass spectrometry analysis of retinal extracts reveals illumination time-dependent accumulation of disulfide homodimers of recoverin and its higher order disulfide cross-linked species, including a minor fraction of mixed disulfides with intracellular proteins (tubulins, etc.). Meanwhile, monomeric bovine recoverin remains mostly reduced. These effects are accompanied by accumulation of disulfide homodimers of visual arrestin. Histological studies demonstrate that the light-induced oxidation of recoverin and arrestin occurs in intact retina (illumination for 2 h), while illumination for 5 h is associated with damage of the photoreceptor layer. A comparison of ex vivo levels of disulfide homodimers of bovine recover...Continue Reading

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Citations

Jan 5, 2016·Journal of Biomolecular Structure & Dynamics·Alexei S KazakovSergei E Permyakov
Jun 24, 2016·Cell Biochemistry and Biophysics·Eugene A PermyakovSergei E Permyakov
Dec 28, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E Yu ZerniiI I Senin
Mar 10, 2017·Chemistry : a European Journal·Melanie WallischJens Christoffers
Mar 18, 2019·Biochemistry. Biokhimii︠a︡·V E BaksheevaI I Senin
Jan 9, 2019·Frontiers in Molecular Neuroscience·Philipp O TsvetkovEvgeni Yu Zernii
Jan 23, 2019·Frontiers in Molecular Neuroscience·Evgeni Yu ZerniiSergei E Permyakov
Nov 22, 2017·Oxidative Medicine and Cellular Longevity·Evgeni Yu ZerniiIvan I Senin
Dec 24, 2018·Antioxidants·Viktoriia E BaksheevaEvgeni Yu Zernii
Nov 20, 2018·Frontiers in Molecular Neuroscience·James B Ames
Jan 10, 2021·Biomolecules·Alisa A VologzhannikovaSergei E Permyakov

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