Light-induced formation of partially reduced oxygen species limits the lifetime of photosystem 1-based biocathodes

Nature Communications
Fangyuan ZhaoFelipe Conzuelo

Abstract

Interfacing photosynthetic proteins specifically photosystem 1 (PS1) with electrodes enables light-induced charge separation processes for powering semiartificial photobiodevices with, however, limited long-term stability. Here, we present the in-depth evaluation of a PS1/Os-complex-modified redox polymer-based biocathode by means of scanning photoelectrochemical microscopy. Focalized local illumination of the bioelectrode and concomitant collection of H2O2 at the closely positioned microelectrode provide evidence for the formation of partially reduced oxygen species under light conditions. Long-term evaluation of the photocathode at different O2 concentrations as well as after incorporating catalase and superoxide dismutase reveals the particularly challenging issue of avoiding the generation of reactive species. Moreover, the evaluation of films prepared with inactivated PS1 and free chlorophyll points out additional possible pathways for the generation of oxygen radicals. To avoid degradation of PS1 during illumination and hence to enhance the long-term stability, the operation of biophotocathodes under anaerobic conditions is indispensable.

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Citations

Oct 6, 2018·Plant Signaling & Behavior·Kazi Khayrul Bashar
Jan 4, 2020·Chemical Communications : Chem Comm·Yuying GaoCan Li
Feb 24, 2019·Nature Communications·Sai Kishore RaviSwee Ching Tan
Apr 24, 2019·Analytical and Bioanalytical Chemistry·Marco MalferrariStefania Rapino
May 26, 2020·Trends in Biotechnology·Alexandra H Teodor, Barry D Bruce
Mar 20, 2019·Journal of the American Chemical Society·Fangyuan ZhaoFelipe Conzuelo
Aug 25, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Kody D WolfeG Kane Jennings
Aug 3, 2019·ACS Sustainable Chemistry & Engineering·Arianna CherubinMassimiliano Perduca

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Methods Mentioned

BETA
thermal treatment
ion exchange chromatography

Software Mentioned

SPECM

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