Abstract
Rho GTPases play a central role in actin-based cytoskeleton reorganization, and they participate in signaling pathways that regulate gene transcription, cell cycle entry, and cell survival. This study verifies the role of Rac1 during light-induced retinal degeneration. BALB/c mice were exposed to degenerative light stimulus, and their eyes were enucleated immediately or after the mice were kept in the dark for 6, 24, and 48 hours. Retinas were fixed and processed for immunohistochemical analysis. The distribution of Rac1 and its effectors-p21-activated kinases (PAKs) 1, 2, and 3-was studied by immunohistochemistry, whereas the expression of PAKs 3, 4, and 5 mRNA was analyzed by real-time PCR. Rac1 activity was measured using a pull-down assay. In control retinas, Rac1 was mostly observed in photoreceptors, plexiform layers, and Müller glial cells. In light-damaged retinas, some TUNEL-positive photoreceptors upregulated Rac1 expression. Conversely, most of the Rac1-positive cells were TUNEL-positive, mainly in early stages of retinal degeneration. The increase in Rac1 expression was preceded by enhanced Rac1 activity, detectable at the end of the light stimulus and still present 48 hours later. The distribution patterns of PAK1,...Continue Reading
Citations
Sep 12, 2008·Journal of Molecular Histology·Virgínia O CremaMarinilce F Santos
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·S A EremeyevL S Yaguzhinsky
Jul 13, 2013·BMC Neuroscience·David M Sherry, Bradley A Blackburn
Oct 3, 2012·PloS One·Vera PaschonAlexandre Hiroaki Kihara
Feb 2, 2013·PloS One·Severin Reinhard HeynenChristian Grimm
May 24, 2013·PloS One·Érica de SousaAlexandre Hiroaki Kihara
Apr 16, 2013·PloS One·Vera PaschonAlexandre Hiroaki Kihara
Apr 22, 2014·PloS One·Akiko SugaMasayo Takahashi
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masatoshi HarutaPaul A Sieving
Jan 5, 2014·Molecular Neurobiology·Guilherme Shigueto Vilar HigaAlexandre Hiroaki Kihara
Sep 12, 2012·The Journal of Cell Biology·François J-M ChartierPatrick Laprise
Aug 15, 2014·Experimental Neurology·Lais Takata WalterAlexandre Hiroaki Kihara
Oct 6, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Alexandre H KiharaLuiz R G Britto
Jan 21, 2014·Photochemistry and Photobiology·Maria Nathália de Carvalho Magalhães MoraesAna Maria de Lauro Castrucci
Jul 24, 2014·Cell Biology International·Kun HanDa-Liu Min
Sep 23, 2014·The FEBS Journal·Maike KösterKarl-Wilhelm Koch
Oct 25, 2016·Scientific Reports·Viviana GuadagniEnrica Strettoi
Aug 17, 2016·Scientific Reports·Arpad PalfiG Jane Farrar
Oct 16, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·I P LimaC Y I Yan
Jul 4, 2017·PloS One·Michelle LajkoAmani A Fawzi
Aug 30, 2014·The Cochrane Database of Systematic Reviews·Sharon D SolomonBarbara S Hawkins
Nov 2, 2006·Glia·Thomas LiedtkeSolon Thanos
Oct 3, 2018·The European Journal of Neuroscience·Gladys Y-P Ko
Dec 5, 2019·Genes·Peter M J Quinn, Jan Wijnholds
Nov 2, 2019·JCI Insight·Sergio Li CalziMaria B Grant