Light-Inducible MiR163 Targets PXMT1 Transcripts to Promote Seed Germination and Primary Root Elongation in Arabidopsis

Plant Physiology
Pil Joong ChungNam-Hai Chua

Abstract

Expression of many plant microRNAs is responsive to hormones and environmental stimuli, but none has yet been associated with light. Arabidopsis (Arabidopsis thaliana) miR163 is 24 nucleotides in length and targets mRNAs encoding several S-adenosyl-Met-dependent carboxyl methyltransferase family members. Here, we found that miR163 is highly induced by light during seedling de-etiolation as well as seed germination. Under the same condition, its target PXMT1, encoding a methyltransferase that methylates 1,7-paraxanthine, is down-regulated. Light repression of PXMT1 is abolished in a mir163 null mutant, but the repression can be restored to wild-type levels in complementation lines expressing pri-miR163 gene in the mir163 mutant background. During seed germination, miR163 and its target PXMT1 are predominantly expressed in the radicle, and the expression patterns of the two genes are inversely correlated. Moreover, compared with the wild type, mir163 mutant or PXMT1 overexpression line shows delayed seed germination under continuous light, and seedlings develop shorter primary roots with an increased number of lateral roots under long-day condition. Together, our results indicate that miR163 targets PXMT1 mRNA to promote seed ger...Continue Reading

Citations

May 30, 2019·Molecular Plant-microbe Interactions : MPMI·Cuizhu ZhaoHaibin Lu
May 14, 2017·Photochemistry and Photobiology·Carlos E HernandoJulieta L Mateos
Jun 5, 2020·Plants·Gerardo Carrera-CastañoLuis Oñate-Sánchez
Aug 25, 2018·Frontiers in Plant Science·Camila Sánchez-RetuertaRossana Henriques
Jun 27, 2019·Functional Plant Biology : FPB·Zhiqiang LiuYanxi Pei
Dec 16, 2017·PloS One·Xuebin ZhangZinnia H González-Carranza
Oct 19, 2019·International Journal of Molecular Sciences·Barczak-Brzyżek AnnaFilipecki Marcin
Dec 20, 2020·International Journal of Molecular Sciences·Pil Joong ChungJu-Kon Kim
Jun 3, 2021·International Journal of Molecular Sciences·Yu WuGenfa Zhang
Jul 3, 2021·Frontiers in Plant Science·Bhavika TiwariWolfgang Frank
Jul 31, 2021·Plant Communications·Rocío Soledad Tognacca, Javier Francisco Botto
Nov 9, 2018·The Science of the Total Environment·Jin XuVinay Kumar

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